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Toxicity to microorganisms

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Reference
Endpoint:
toxicity to microorganisms
Type of information:
other: Published data
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
equivalent or similar to
Guideline:
other: Environment Canada EPS 1/RM/24: biological test method: toxicity test using luminescent bacteria
Principles of method if other than guideline:
Microtox Toxicity Analyser system, Microbics Corporation (Carlsbad CA). Test substance in added to cells suspensions and light readings are measured before and 15 minutes after sample addition. Light loss is proportional to toxicity of the sample, with 50% light loss being defined as the EC50.
GLP compliance:
not specified
Analytical monitoring:
not specified
Details on sampling:
- concentrations: 10,,20,30,40 mg/l
- Sampling method: 4g of test substance was extracted in 20 ml of saline. This sample was weigehed into 2% NaCl to make a 1% (10g/l) solution. This sample was diluted into a concentration range where a normal dose response was observed with LBT
Vehicle:
no
Test organisms (species):
Photobacterium phosphoreum
Details on inoculum:
- Laboratory culture:B-NRRL 11177
- Initial biomass concentration:50g/l
Test type:
other: closed test vial
Water media type:
saltwater
Limit test:
no
Total exposure duration:
15 min
Test temperature:
15°C
pH:
7.8
Dissolved oxygen:
9.1 mg/l
Salinity:
2% NaCl
Nominal and measured concentrations:
nominal concentrations: 10,,20,30,40 mg/l (recovery: 98-103%)
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable):closed
- No. of organisms per vessel:10µl of cell suspension

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : reduction in light
Reference substance (positive control):
yes
Remarks:
sodium hypochlorite
Duration:
15 min
Dose descriptor:
EC50
Effect conc.:
22 mg/L
Nominal / measured:
estimated
Basis for effect:
other: light reduction
Remarks on result:
other: the luminiscent bacteria toxicity test gives following result: 15 minutes EC50 = 22 mg/L for photobacterium phosphoreum
Validity criteria fulfilled:
not specified
Conclusions:
The luminiscent bacteria toxicity test gives following result: 15 minutes EC50 = 22 mg/L for photobacterium phosphoreum.
When Potassium oxide is hydrated is produced Potassium hydroxide. Therefore, the health effects of Potassium hydroxide need to be considered in the assessment of Potassium oxide

Description of key information

The luminiscent bacteria toxicity test gives following result: 15 minutes EC50 = 22 mg/L for photobacterium phosphoreum.

Key value for chemical safety assessment

EC50 for microorganisms:
22 mg/L
EC10 or NOEC for microorganisms:
4.4 mg/L

Additional information

The luminiscent bacteria toxicity test gives following result: 15 minutes EC50 = 22 mg/L for photobacterium phosphoreum.