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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Study period:
1970-05-01
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: This study is classified as reliable with restrictions because there was no GLP statement provided, and limited data on methods were reported, but the study seemed to be well-conducted.

Data source

Reference
Reference Type:
publication
Title:
The Toxicological Properties of Hydrocarbon Solvents
Author:
Hine, CH, and Zuidema, HH
Year:
1970
Bibliographic source:
Industrial Medicine, Vol. 39, No. 5, May 1970.

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
GLP compliance:
no
Test type:
standard acute method

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): S-1
- Composition of test material, percentage of components: C-6 normal and iso paraffins (hexanes) and naphthenes (methyl-cyclohexane, dimethylcyclohexane), 25-35% n-hexane

Test animals

Species:
rat
Strain:
Long-Evans
Sex:
male
Details on test animals and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 150-300 g

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
not specified
Vehicle:
other: unchanged (no vehicle)
Details on inhalation exposure:
GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure chamber volume: 250 l
- Method of conditioning air: Sample was heated in a water bath at 77 degree F. Air was bubbled through the sample and entered the exposure chamber. For concentrations less than saturation, air flow was divided with part passing over the sample and part passing directly into the exposure chamber.

TEST ATMOSPHERE
- Brief description of analytical method used: GLC

Analytical verification of test atmosphere concentrations:
not specified
Duration of exposure:
4 h
Concentrations:
73,680 ppm (30-40% of saturation at 25 degree C)
81,800 ppm
No. of animals per sex per dose:
10 males
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: at least 6 days

Results and discussion

Effect levels
Sex:
male
Dose descriptor:
LC50
Effect level:
73 860 ppm
Exp. duration:
4 h
Remarks on result:
other: 259354 mg/m3
Mortality:
All deaths occurred during exposure, except one rat in the 81800 ppm group that died on day 6.
Clinical signs:
Surviving rats were uncoordinated, prostrate or comatose during exposure but recovered within a few hours of removal from the chamber. The rat that died on day 6 had convulsions during and after exposure.

Applicant's summary and conclusion

Interpretation of results:
study cannot be used for classification
Remarks:
Migrated information
Conclusions:
The 4-hr LC50 for rats exposed by inhalation was 73,680 ppm (259354 mg/m3).
Executive summary:

This study examined that acute inhalation toxicity of hexane to male rats. Groups of 10 male rats were exposed to various large concentrations of hexane vapor for 4 hrs. Animals were then observed for clinical signs and mortality for at least the next 6 days. Several animals died during the exposure period. Surviving animals experienced severe toxicological effects during the exposure. One animal experienced convulsions during and after exposure, and died on day 6 post-exposure. The LC50 was determined to be 73,680 ppm (259354 mg/m3). Due to the high concentration of the LC50, the test substance would not be classified as toxic by inhalation according to OECD GHS guidelines.