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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Eye irritation

Currently viewing:

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1971
Report date:
1971

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
Principles of method if other than guideline:
BASF-Test
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2,2',2''-nitrilotriethanol
EC Number:
203-049-8
EC Name:
2,2',2''-nitrilotriethanol
Cas Number:
102-71-6
Molecular formula:
C6H15NO3
IUPAC Name:
2-[bis(2-hydroxyethyl)amino]ethan-1-ol
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: No. XXI/170


Test animals / tissue source

Species:
rabbit
Strain:
Vienna White
Details on test animals or tissues and environmental conditions:
TEST ANIMALS
- Mean Weight at study initiation: 2.45 kg
- Diet: Altromin MS (Altroge, Germany) ad libitum
- Water: ad libitum


Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: concurrent saline
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 50 m³
- Concentration: concentrated
Duration of treatment / exposure:
not applicable (substance was not washed out)
Observation period (in vivo):
8 days
Number of animals or in vitro replicates:
2 (m/f)
Details on study design:
REMOVAL OF TEST SUBSTANCE
- Washing: none

Readings at 10 min, 1 and 24 hrs, 3 and 8 days

SCORING SYSTEM: initial scoring system was converted into Draize-scores

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: 1, 24 hours
Score:
1
Reversibility:
fully reversible within: 8 days
Remarks on result:
other: redness
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: 8 days
Score:
0
Reversibility:
fully reversible within: 8 days
Remarks on result:
other: redness
Irritation parameter:
chemosis score
Basis:
mean
Time point:
other: 1 hour
Score:
1
Reversibility:
fully reversible within: 8 days
Irritation parameter:
chemosis score
Basis:
mean
Time point:
other: 24 hours, 8 days
Score:
0
Reversibility:
fully reversible within: 8 days

Any other information on results incl. tables

Readings

Animal

Cornea

Iris

Conjunctiva

Additional findings

op

ar

red

ch

di

1 h

1

-

-

-

1

1

-

 

2

-

-

-

1

1

-

 

24 h

1

-

-

-

1

0

-

 

2

-

-

-

1

0

-

 

48 h

1

 

 

 

 

 

 

 

2

 

 

 

 

 

 

 

72 h

1

 

 

 

 

 

 

 

2

 

 

 

 

 

 

 

8 d

1

-

-

-

0

0

-

 

2

-

-

-

0

0

-

 

Mean

24 -72 h

1

 

 

 

 

 

 

 

2

 

 

 

 

 

 

 

Mean

24-72 h

1-2

 

 

 

 

 

 

 

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met