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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Description of key information

With high probability 2,2',2"-nitrilotriethanol has no chronic effects to aquatic invertebrates.

Key value for chemical safety assessment

EC10, LC10 or NOEC for freshwater invertebrates:
16 mg/L

Additional information

To determine the chronic toxicity of 2,2',2"-nitrilotriethanol to aquatic invertebrates, a non-GLP reproduction toxicity study was performed using Daphnia magna as test species. The test followed a method of the German Federal Environmental Agency which is similar to the OECD TG 211. The test was designed as a semi-static procedure with closed vessels and exposure period of 21 days. Test concentrations were analytically verified and deviated less than 20% from nominal concentrations. Parental mortality, number of offspring per animal and first appearance of offspring were determined. The most sensitive endpoint was parental mortality. The 21 -d NOEC for parental mortality was determined to be 16 mg/L. The 21 -d NOEC for reproduction was 125 mg/L, the NOEC for appearance of first offspring was 250 mg/L (Kuehn et al./UBA 1989; Kuehn et al., 1988).

This effect value is supported by the ACR approach (ECETOC, 2003). ECETOC developed the Acute to Chronic Ratio (ACR) based on toxicity data from various tests with different aquatic species (fish and aquatic invertebrates). Depending on the applied pooling criteria ACR values (90%-ile) have been calculated for different aspects. The relevant ACRs have a range from 11.5 (narcotic mode of action) to 94.5 (substance data for individual species). As the key value for triethanolamine is rather high (48-h EC50 = 610 mg/L, Warne et al., 1996), the NOEC is expected to be larger than 1 mg/L. Therefore, it can be concluded that chronic effects are not expected.