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Toxicological information

Skin sensitisation

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Administrative data

Endpoint:
skin sensitisation: in vivo (LLNA)
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
Data are lacking in the publication but the study has been performed by the team that developped the test. So it is considered that it has been appropriately conducted. However, as DES is corrosive, and LLNA is known to induce false positive response for very irritant substances, the validity of this positive response is doubtful.

Data source

Reference
Reference Type:
publication
Title:
Structure activity relationships in skin sensitization using the murine local lymph node assay
Author:
Ashby J, Basketter DA, Paton D, Kimber I
Year:
1995
Bibliographic source:
Toxicology 103: 177-194

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
GLP compliance:
not specified
Type of study:
mouse local lymph node assay (LLNA)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
no further data

In vivo test system

Test animals

Species:
mouse
Strain:
not specified
Sex:
not specified

Study design: in vivo (LLNA)

Vehicle:
acetone/olive oil (4:1 v/v)
Concentration:
1; 2.5; 10 %
No. of animals per dose:
no data

Results and discussion

In vivo (LLNA)

Resultsopen allclose all
Parameter:
SI
Remarks on result:
other: 0.8 - 1.9 and 12.0 for 1; 2.5 and 10% respectively
Parameter:
other: disintegrations per minute (DPM)
Remarks on result:
other: no data

Any other information on results incl. tables

no further data

Applicant's summary and conclusion

Interpretation of results:
sensitising
Remarks:
Migrated information
Conclusions:
DES is positive in this LLNA test
Executive summary:

A LLNA test has been perfomed with DES as one of the 106 chemicals tested. A SAR was developed based on the electrophilicity of the substance.

DES was considered as a sensitizer in this test.