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Ecotoxicological information

Toxicity to terrestrial plants

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Administrative data

Endpoint:
toxicity to terrestrial plants: long-term
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: No LD50 or NOEC determined because only 1 concentration was tested. Very few details are available in this short note.

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
MUTATION STUDIES WITH DIETHYL SULFATE (DES)
Author:
Corcos A
Year:
1981
Bibliographic source:
Arabidopsis inf serv 18: 150-152
Reference Type:
publication
Title:
Further mutation studies with Diethyl sulfate (DES)
Author:
Corcos A
Year:
1982
Bibliographic source:
Arabidopsis inf serv 19: 123

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Exposure of seeds to one concentration of DES and the growth and survival of the plants are followed up to the production of seeds and germination of this second generation.
In the second publication, the same study was repeated with 0.15% of DES.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
no data

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
no

Test organisms

Species:
other: Arabidopsis thaliana
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
- Common name: thale cress
- Plant family: Brassicaceae
- Variety: Columbia
- Source of seed: no data
- Prior seed treatment/sterilization: no data
- Historical germination of seed (germination of seed lot tested): no data
- Seed storage: no data

Study design

Test type:
other: whole life cycle experiment
Study type:
laboratory study
Substrate type:
not specified
Limit test:
no
Remarks:
no data

Results and discussion

Effect concentrationsopen allclose all
Species:
other: Arabidopsis thaliana
Dose descriptor:
other: effect at the only concentration tested
Effect conc.:
0.5 other: %
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: germination, growth, flowering, seed production and germination of the M2 generation
Species:
other: Arabidopsis thaliana
Dose descriptor:
other: effect at the only concentration tested
Effect conc.:
0.15 other: %
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: seed germination of the M2 generation

Any other information on results incl. tables

Germination was not affected by the treatment since 111 out of 114 seeds germinated (97.3%). Among the control, 40 out of 40 germinated.

The control plants flowered 19 days after germination. So did 38 of the treated plants (33.3% of the treated seeds), however, 71 (62.3% of the treated seeds) did not flower by that time and 5 had died (4%).

The control plants produced seeds 56 days after germination. So did the 38 non delayed plants. Among the delayed ones, 24 had flowered by that time (33.8% of the delayed plants), but the plants were small. The other plants died without flowering (66.2% of the delayed plants).

Germination of the M2 plants has been seriously affected by the treatment with DES (Table 1). The size of the M2 plants was in general smaller than the controls. Only among the progeny of M1 plants 4,5,and 6 were normal sized plants.

Table 1: Germination results of M2 plants (exposure at 0.5%)

M1

numbers

%

Plant 1

13 out of 40

32.5

Plant 2

15 out of 40

37.5

Plant 3

15 out of 40

37.5

Plant 4

11 out of 40

27.5

Plant 5

27 out of 40

64.5

Plant 6

25 out of 40

62.5

Table 2: Germination results of M2 plants (exposure at 0.15%)

M1

numbers

%

Plant 1

8 out of 20

40

Plant 2

3 out of 10

30

Plant 3

9 out of 20

45

Plant 4

4 out of 20

20

Plant 5

4 out of 20

20

Plant 6

6 out of 20

30

Plant 7

5 out of 20

25

Plant 8

4 out of 20

20

Plant 9

4 out of 20

20

Plant 10

7 out of 24

29

Plant 11

6 out of 43

14

Plant 12

1 out of 10

10

Plant 13

6 out of 36

16

Plant 14

23 out of 43

53

Plant 15

4 out of 14

28

Plant 16

4 out of 9

44

Plant 17

3 out of 7

42

Plant 18

1 out of 6

16

Plant 19

6 out of 18

33

Total

108 out of 333

32

Applicant's summary and conclusion

Validity criteria fulfilled:
not applicable
Remarks:
Not a standard study
Conclusions:
The drenching of seeds in DES solution at 0.5% induced effects on survival, plant growth, flowering time and seed production and germination of the second generation. The repetition of the study with 0.15% had the same effect on the germination of the M2 plants.
Executive summary:

Seeds of Arabidopsis thaliana were exposed to DES (0.5%) and then grown. Survival, germination, growth, flowering time and seed production were determined. Seeds from some of these plants were then reexposed to DES and germination was determined.

DES produced effects on survival, plant growth, flowering time and seed production of the M1 generation and also decreased the germination rate of M2 generation (average germination: 44%).

The repetition of the study with 0.15% had the same effect on the germination of the M2 plants (32%).