Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
other information
Study period:
01-DEC-1977 to 10-APR-1978
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Old study but performed by the Japanese Authorities according to national guidelines

Data source

Reference
Reference Type:
other: CERI website
Title:
Diethyl sulfate
Author:
Anonymous
Bibliographic source:
CERI website

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: Japanese guideline before OECD harmonisation
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
purity: 99%
Impurities and water < 0.15%

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Duration of test (contact time):
4 wk
Initial test substance concentration
Initial conc.:
100 mg/L
Based on:
not specified

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (TOC removal)
Value:
51.2
Sampling time:
28 d
Parameter:
% degradation (TOC removal)
Value:
95.9
Sampling time:
28 d
Parameter:
other: BOD
Value:
49.1
Sampling time:
28 d
Parameter:
other: BOD
Value:
89.1
Sampling time:
28 d
Details on results:
No mean was calculated because the results of the 2 replicates are very different.
Preliminary test:
after 24h, no residue of DES was found in the water or in the sludge.
Based on TOC analysis, the recovery was 99% in water and 95 % in the sludge.
GC analysis shows that ethanol was a degradation product.

Main test results:
Based on the results of TOC, BOD and GC analysis, the following reaction is supposed to occur:
(C2H5O)2 SO2 ---> H2O + C2H5OSO3H + C2H5OH ---> H2O + H2SO4 + 2(C2H5OH)
Hydrolysis plays a role in this degradation.

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Remarks:
the validity criteria of the guideline used are unknown
Interpretation of results:
other: Quickly biodegraded but no data about the 10-day window. Abiotic degradation is also included
Conclusions:
Quickly biodegraded but no data about the 10-day window. Abiotic degradation is also included.
Executive summary:

The Japanese Authorities performed a study of biodegradation of DES with activated sludge.

Results show that DES is quickly biodegraded but no data is given about the 10-day window. Abiotic degradation is also included.

Ethanol was quantified as a degradation product.

The results of the 2 replicates are rather different.