Registration Dossier

Administrative data

Endpoint:
additional toxicological information
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: no guideline followed, but detailed publication

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1996

Materials and methods

Type of study / information:
Using IGEC biotest (inhibition of growth of Escherichia coli) and acetylcholinesterase (AChE) inhibition tests to evalutate the toxicity of DMA (and other dithiocarbamates) by in vitro application.
Principles of method if other than guideline:
The IGEC biotest was used to determine the minimal effective concentration (MEC), median effective concentration (EC50), and no observed effect concentration (NOEC). Additionally, the AChE inhibition test was carried out for all tested substances.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Dimethylamine (Aldrich); no further details given.

Results and discussion

Any other information on results incl. tables

IGEC biotest

For dimethylamine the following results were reported:

MEC = 10 µg/mL

EC50 = 10.085 µg/mL

NOEC = 3779 µg/mL

Taking this results into account dimethylamine was the least toxic amine analyzed in this study.

Acetylcholinesterase Inhibition Test

The results which were obtained in this study indicate a generalized absence of AChE inhibition for dimethylamine and all other tested dithiocarbamates.

Applicant's summary and conclusion

Conclusions:
Dimethylamine showed no toxicity effects using the IGEC biotest and no inhibition properties on the enzyme acetylcholinesterase.
Executive summary:

Taking the results of the IGEC biotest into account dimethylamine was the least toxic amine which was analyzed in this study. Additionally no inhibition on the enzyme acetylcholinesterase was indicated.