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Ecotoxicological information

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The acute toxicity of e-Caprolactone to guppies (Poecilia reticulata) was tested according to OECD guideline 203 at nominal test concentrations of 1000, 500, 250, 125, 62, 31 and 0 mg/L in ISO-water. The 96 hour LC50 was 280 mg/L. The NOEC for mortality and other effects was 250 mg/L (Groeneveld et al, 1992).

The toxicity of e-Caprolactone to the water flea, Daphnia magna, was determined in the 48 hour acute immobilisation test conducted according to OECD 202. The daphnids were exposed to concentrations of e-Caprolactone between 6.25 and 1000mg/l. The 48 hour EC50 was 204mg/L (with 95% confidence limits: 172 -240mg/L) (Hisgen, 2003).

Desmodesmus subspicatus was exposed to e-Caprolactone at nominal concentrations of 102, 256, 640, 1600 and 4000 mg/L in a static 72 -hour toxicity test carried out according to OECD 201 guidelines. The test design included an untreated control comprising untreated algal growth medium only. Concentrations of E-Caprolactone were verified analytically at test initiation and termination and measured concentrations were 91 - 106% of nominal. The 72 -hour ErC50 and EbC50 endpoints determined were 2616 and 1217 mg/L, respectively. The corresponding 72 -hour NOEC based on inhibition of specific growth rate and inhibition of biomass were both 256 mg/L, respectively (Werner, 2003).

The toxicity of e-Caprolactone toPseudomonas putidawas tested in a series of studies. The results are based on calculated concentrations. Significant inhibition of cell multiplication occurred at calculated test concentrations of 63mg/L and higher. The EC50 was calculated to be 1260mg/L and the corresponding NOEC was 32mg/L (Jansen & van den Berg, 1992).

In the chemical safety assessment performed according to Article 14(3) in connection with Annex I section 3 (Environmental Hazard Assessment) no hazard was identified. Therefore according to REACH Annex I (5.0) exposure estimation is not necessary for long-term toxicity to fish and aquatic invertebrates. Consequently, in accordance with Column 2 of REACH Annex IX, the study does not need to be conducted as all identified uses of the substance are assessed as safe for the environment