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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

The acute toxicity of the test item to the freshwater invertebrate Daphnia magna has been investigated and gave a 48-Hour EC50 value of 8.3 mg/l with 95% confidence limits of 6.9  - 10 mg/l.  The No Observed Effect Concentration at 48 hours was 3.2 mg/l.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
8.3 mg/L

Additional information

Introduction

A study was performed to assess the acute toxicity of the test item to Daphnia magna. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (April 2004) No 202, "Daphnia sp, Acute Immobilisation Test" referenced as Method C.2 of Commission Regulation (EC) No. 440/2008.

Methods.

Following a preliminary range-finding test, twenty daphnids (2 replicates of 10 animals) were exposed to an aqueous solution of the test item at concentrations of 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/l for 48 hours at a temperature of 20 to 22°C under static test conditions. The number of immobilised Daphnia were recorded after 24 and 48 hours.

Results

The 48-Hour EC50 for the test item to Daphnia magna based on nominal test concentrations was 8.3 mg/l with 95% confidence limits of 6.9 - 10 mg/l. The No Observed Effect Concentration was 3.2 mg/l.

Analysis of the test preparations at 0 and 48 hours showed measured test concentrations to range from 80% to 117% of nominal value and so the results are based on nominal test concentrations only.