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EC number: 236-337-7
CAS number: 13308-51-5
The influence of Boron orthophosphate (CAS 13308-51-5) on growth
of the uni-cellular freshwater green alga Pseudokirchneriella
subcapitata was investigated in a static OECD 202 test under GLP
conditions. Exponentially-growing cultures of the green alga were
exposed to various concentrations of Boron orthophosphate over several
generations under defined conditions for 72 hours. Since the test item
is of low water solubility, the highest nominal test concentration was
prepared following the Water Accommodated Faction (WAF) approach
according to the OECD guidance documents No. 23 and 29 to obtain the
maximal solubility of the test item under the test conditions. The algae
were exposed to a series of dilutions obtained from a filtrated eluate
of a 100 mg/L of loading. The media preparation was performed under
For the determination of alga growth eight replicates of the
control (test medium only) and four replicates of each test
concentration were exposed to theoretical loadings of:
Nominal test item loadings: Control, 26, 36.4, 51.0, 71.4 and 100
mg/L, equivalent to theoretical nominal boron loading: Control, 2.65,
3.71, 5.20, 7.28 and 10.19 mg B/L.
The concentrations of boron in the test media were determined by
chemical analyses in the aqueous phase at the start and at the end of
the test using ICP-OES (LOQ 0.557 μg B/L).
At test start, the measured boron concentrations were 0.336,
0.475, 0.679, 0.968 and 1.35 mg/L (between 12.7 and 13.3 % of nominal
loading). The boron concentrations were stable at all test substance
concentrations throughout the test period (101 - 104 % compared to the
measured initial concentrations). The test evaluation was based on the
measured initial concentrations of boron due to the stability of the
test item throughout the test, as well as on the nominal test substance
There were no concentration-dependent effects on the growth of the
green alga up to the highest test concentrations of 1.35 mg boron/L. The
ErC50 and EyC50 values for inhibition of growth rate and yield were set
to be higher than the highest test concentration of 100 mg/L nominal.
The NOEC for both, growth rate and yield was ≥ 100 mg/L nominal
test substance condition, corresponded to 1.35 mg boron/L (measured
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