Citronellal

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study meets basic scientific principles, sufficiently documented

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Test guideline

Qualifier:

no guideline followed

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Citronellal

EC Number:

203-376-6

EC Name:

Citronellal

Cas Number:

106-23-0

Molecular formula:

C10H18O

IUPAC Name:

3,7-dimethyloct-6-enal

Details on test material:

Name of test material (as cited in study report): Citronellal (78/742)

Test animals

Species:

mouse

Strain:

NMRI

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Wiga
- Weight at study initiation: 22-28 g (male); 22-24 g (female)
- Fasting period before study: 15 -20 hours prior to application

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

CMC (carboxymethyl cellulose)

Doses:

200, 700 mg/kg

No. of animals per sex per dose:

5

Details on study design:

- Duration of observation period following administration: 15 days
- Frequency of observations: 15 min, 30 min, 1 h, 2 h, 4 h, 5 h, d 1-9, d 12-15; weighing: before exposure, d 2-4, d 7, d 13
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Statistics:

Statistic test on significance performed (no further details given)

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 200 - < 700 mg/kg bw

Mortality:

700 mg/kg: male: (5/5) day 1; female: (5/5) day 1;
200 mg/kg: male: (0/5) day 15; female: (0/5) day 15

Clinical signs:

see Table 1

Body weight:

see Table 2

Gross pathology:

no intra-abdominal precipitations or adhesions; no further findings were reported

Any other information on results incl. tables

Table 1: Clinical signs

Dose (mg/kg)	700	200
dyspnoea	***	***
apathy	***	***
abnormal position	***
tumbling	***	***
atony	***
twitches	***
spasmic movement	***
Saltation convulsions	***
clonic cramps	***
erythema	***
exsicosis	***
overbalance	***
disturbance of equilibrium	***
paresis	***
poor general condition	***	***

Table 2: Mortality and body weight gain

Dose (mg/kg)	700*		200*
sex	male	female	male	female
number of animals	5	5	5	5
dead animals after 1 hour	1	1	0	0
1 day	5	5	0	0
2 days	5	5	0	0
7 days	5	5	0	0
15 days	5	5	0	0
mean body weight gain (g)	-	-	7.2	4.8

* used for LD₅₀ calculation

LD₅₀ > 200 mg/kg (statistical significance: 1%)

LD₅₀ < 700 mg/kg (statistical significance: 1%)

observation period 15 days (male and female animals)