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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1982
Report date:
1982

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
GLP compliance:
no
Remarks:
study performed prior to implementation of GLP
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
1-chlorohexane
EC Number:
208-859-5
EC Name:
1-chlorohexane
Cas Number:
544-10-5
Molecular formula:
C6H13Cl
IUPAC Name:
1-chlorohexane

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Weight at study initiation: males 215 g, females 148 g
- Housing: 1-5 animals per cage (Makrolon type III)
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: 4-8 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20°C +/- 1°C
- Humidity (%): 60% +/- 5%
- Air changes (per hr): 15/h
- Photoperiod (hrs dark / hrs light): 12/12

IN-LIFE DATES: From: 26.07.1982 To: 28.07.1982

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
paraffin oil
Details on oral exposure:
DOSE VOLUME APPLIED: 10 ml/kg
Doses:
1990, 3980, 5010, 6310, 7940, 8780 mg/kg bw
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations: observation up to 6 hours after application and daily thereafter
- Frequency of weighing: before application and 1, 7 and 14 days thereafter
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight
Statistics:
LD50 value was determined according to Litchfield and Wilcoxon (J. Pharmacol. Exp. Ther. 96, 1949, 99).

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
7 000 mg/kg bw
Based on:
test mat.
95% CL:
5 623 - 9 310
Mortality:
1990 mg/kg: 0/5 females, 0/5 males
3980 mg/kg: 2/5 females, 0/5 males
5010 mg/kg: 3/5 females, 0/5 males
6310 mg/kg: 2/5 females, 1/5 males
7940 mg/kg: 4/5 females, 1/5 males
8780 mg/kg: 5/5 females, 4/5 males

Deaths occured within 7-96 h after application. 
Clinical signs:
other: All animals were affected. 1 h p.a. piloerection, 2 h p.a. ataxia, lateral position and sedation occured, that were reversible within 48-96 h. 
Gross pathology:
Liver congestion and blood in the small intestine was observed in several animals. No substance related macroscopic findings in surviving animals.

Applicant's summary and conclusion

Interpretation of results:
study cannot be used for classification
Remarks:
Migrated information
Conclusions:
In this acute oral toxicity study in the rat a combined LD50 value of 7000 mg/kg bw was found. Acute clinical signs were observed in all dose groups which were reversible within 48-96 h p.a. .