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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
No data
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Klimish 2f Study was performed using an accepted calculation method

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Composition of the essential oil of Lepidium meyenii (Walp.)
Author:
Tellez M.R., Khan I.A., Kobaisy M., Schrader K., Dayan F.E. and Osbrink W.
Year:
2002
Bibliographic source:
Phytochemistry, 61(2), 149 - 155
Reference Type:
other: Databank
Title:
No information
Author:
Beilstein
Year:
2010
Bibliographic source:
Beilstein database, CAS n° 140-29-4

Materials and methods

Principles of method if other than guideline:
Rapid bioassay utilizing 96-well cell culture plates for screening herbicides and other phytotoxins for selective toxicity toward cyanobacteria and algae (according to Schrader et al. (1997))
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report):phenylacetonitrile
No more data

Sampling and analysis

Analytical monitoring:
not specified
Details on sampling:
No data

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): pentane
No more data

Test organisms

Test organisms (species):
other: Oscillatoria perornata and Selenastrum capricornutum
Details on test organisms:
No data

Study design

Test type:
not specified
Water media type:
not specified
Limit test:
no
Total exposure duration:
4 d
Post exposure observation period:
No data

Test conditions

Hardness:
No data
Test temperature:
No data
pH:
No data
Dissolved oxygen:
No data
Salinity:
No data
Nominal and measured concentrations:
0.0117, 0.117, 1.17, 11.7 and 117 µg/mL
Details on test conditions:
No data
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
1 d
Dose descriptor:
other: Lowest Complete Inhibition Concentration (LCIC)
Effect conc.:
117 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
other: Absorbance at 650 nm
Remarks on result:
other: Oscillatoria perornata
Duration:
4 d
Dose descriptor:
NOEC
Effect conc.:
117 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
other: Absorbance at 650 nm
Remarks on result:
other: Selenastrum capricornutum
Details on results:
No data
Reported statistics and error estimates:
No data

Applicant's summary and conclusion

Validity criteria fulfilled:
not applicable
Conclusions:
Under the test conditions a lowest complete inhibition concentration (LCIC) of 117 µg/mL was found after 1 day for the cyanobacterium Oscillatoria perornata, whilst a NOEC of 117 µg/mL was observed after 4 days for the green alga Selenastrum capricornutum. Thus, phenylacetonitrile may be a selective growth inhibitor for Oscillatoria perornata, but based on these results the substance cannot be classified according to the CLP regulation (EC) N° 1272/2008.
Executive summary:

The authors tested the toxicity of phenylacetonitrile (CAS n° 140-29-4) to the cyanobacterium Oscillatoria perornata and the green alga Selenastrum capricornutum in a 4d growth inhibition test. This test was a rapid bioassay test according to Schrader et al. (1997) which utilizes 96-well cell culture plates for screening substances for selective toxicity toward cyanobacteria and algae by measuring the absorbance at 650 nm. The concentrations tested were 0.0117, 0.117, 1.17, 11.7 and 117 µg/mL. Total exposure duration was 4d and every 24h the absorbance was measured of the different samples.

Under these test conditions, a lowest complete inhibition concentration (LCIC) of 117 µg/mL was found after 1 day for Oscillatoria perornata, whilst a NOEC of 117 µg/mL was observed after 4 days for Selenastrum capricornutum. In conclusion, phenylacetonitrile is selectively toxic towards Oscillatoria perornata compared to Selenastrum capricornutum. Thus, phenylacetonitrile may be a selective growth inhibitor for Oscillatoria perornata, but based on these results the substance cannot be classified according to the CLP regulation (EC) N° 1272/2008.