Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report Date:
1988

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
Cited as Directive 84/449/EEC, C.2 (Acute toxicity for Daphnia)
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): N,N'-Dimethylharndstoff (N,N'-Dimethylurea)
Water solubility: > 500 mg/l at 20 °C

Sampling and analysis

Analytical monitoring:
no

Test solutions

Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test medium of the highest test concentration, 500 mg/l (nominal) was prepared. Following this, serial dilutions of this test medium were prepared in order to obtain the desired test concentrations. Additionally, a culture medium control was tested in parallel.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: originally obtained from a culture received from the Institut National de Recherche Chimique Apliquee, France in 1978. Bred at the testing facility since 1978
- Age at study initiation (mean and range, SD): < 24 h
ACCLIMATION
- Type and amount of food:yeast and green algae

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.80 mmol/l
Test temperature:
19 °C - 21 °C
pH:
8.0 (at study begin); 7.7 - 7.9 after 48 hours
Dissolved oxygen:
8.5 - 8.7 mg/l (at study begin); 8.0 after 48 hours
Nominal and measured concentrations:
Nominal test concentrations:
0, 31.25, 62.5, 125, 250 and 500 mg/l.
Details on test conditions:
TEST SYSTEM
- Test vessel: reagent tubes with flat bottom
- Fill volume: 10 ml
- Aeration: yes
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: charcoal filtered, unchlorinated tap water
- Na/K ratio: 10 : 1
- Acidity: constant - pH 4.3: 0.80 +/- 0.1 mmol/l
- Ca/mg ratio: 4 : 1
- Conductivity: 550 - 650 µS/cm


OTHER TEST CONDITIONS
- Adjustment of pH: with H2SO4
- Photoperiod: 16:8 hours day-night regime
- Light intensity: 5 µE at a wave length of 400 - 750 nm

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 3, 6, 24 and 48 hours.

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

Results after 24 h:
EC0 = 500 mg/l
EC50 > 500 mg/l
EC100 > 500 mg/l

Applicant's summary and conclusion