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EC number: 810-418-2 | CAS number: 1703784-30-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Apr 2019 to Sep 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- 2010
- Deviations:
- no
- Principles of method if other than guideline:
- NA (study was performed according to guideline).
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Reaction products of acrylonitrile and 2,2’-Iminodi(ethylamine), hydrogenated
- EC Number:
- 810-418-2
- Cas Number:
- 1703784-30-8
- IUPAC Name:
- Reaction products of acrylonitrile and 2,2’-Iminodi(ethylamine), hydrogenated
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- PU-2018-821 (batch number: 2298695)
Test item PU-2018-821
Trade name Ortegol LA 2
Batch number 2298695
CAS No. 1703784-30-8
CAS name 2-propenenitrile, reaction products with diethylenetriamine, hydrogenated
Composition: 100%
Molecular weight: 217 g/mol
Molecular formula: C10H27N5
Appearance Colorless - light yellowish liquid
Density 0.96 g/cm³
pH 9 - 10
Water solubility Soluble
Expiry date: 2019-12-31
Recommended storage Room temperature, protection from light, under nitrogen in the tightly closed container
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- Measurement of the Respiration Rates:
After 3 h incubation, the oxygen concentration of each control, reference item and test item replicate was determined by means of oximeter every 5 seconds after transferring the solution to the measuring chamber and the oxygen depletion was recorded for at least 5 minutes with the software PreSens Measurement Studio 2.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: All concentrations were diluted from a stock solution.
- Controls: Test medium without test or reference item. Test medium without test or reference item with ATU
- Other relevant information: The reference item N-Methylanilin was tested with three concentrations within the concentration range 0.1 – 1.8 – 32 mg/L and a dilution factor of 18 (with and without ATU).
The reference item Copper (II)-sulfate pentahydrate was tested with three concentrations within the concentration range 58 - 180 mg/L and a dilution factor of approximately 1.8 (without ATU).
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- A mixed population of activated sewage sludge micro organisms was obtained on 08 April 2019 from the aeration stage of the Municipal sewage treatment plant of 31137 Hildesheim, Germany which treats predominantly domestic sewage.
Preparation of Inoculum:
The sludge was washed twice and adjusted with chlorine free tap water if necessary. The sludge was used within 24 h after sampling.
Dry sludge concentration 3.21 g/L, corresponding to 1.61 g/L in the test vessel for replicates without ATU and 3.19 g/L, corresponding to 1.60 g/L in the test
vessel for replicates with ATU.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Remarks on exposure duration:
- NA
- Post exposure observation period:
- NA
Test conditions
- Hardness:
- Not specified
- Test temperature:
- 20°C
- pH:
- 7.26 to 8.57
- Dissolved oxygen:
- 5.91 to 8.19 mg O2/L (>60% dissolved oxygen saturation in all vessels)
- Salinity:
- NA
- Conductivity:
- Not specified
- Nominal and measured concentrations:
- Nominal concentrations: 4 - 16 - 64 - 256 - 1000 mg/L
- Details on test conditions:
- Based on the results of the preliminary test (see Annex I for details) the definitive study was carried out with 5 concentrations within the
range of 4 to 1000 mg/L (with and without ATU) in a geometrical series and with a dilution factor of 4. Additionally, replicates with
1000 mg/L were pH adjusted with 1m HCL. All concentrations were diluted from a stock solution.
Triplicates of the control (without ATU) without test item were included at the beginning and at the end of the replicates without ATU.
Triplicates of the control (with ATU) without test item were included at the beginning of the replicates with ATU and at the end of the test.
The reference item Copper (II)-sulfate pentahydrate was tested with three concentrations within the concentration range 58 - 180 mg/L
and a dilution factor of approximately 1.8 (without ATU).
The reference item N-Methylanilin was tested with three concentrations within the concentration range 0.1 – 1.8 – 32 mg/L
and a dilution factor of 18 (with and without ATU).
A fresh sample of activated sludge taken from the sewage plant Hildesheim was used for the test procedure. It was washed twice
and adjusted with chlorine free tap water to a dry sludge concentration of 3.0 g/L ± 10 %.
pH The pH value of the activated sludge was determined prior to test start. The pH value of the synthetic waste water was determined
prior to use. Adjustment to 7.5 ± 0.5 was not necessary.
Oxygen After 3 h incubation, the oxygen concentration of each control, reference item and test item replicate was determined by means of
oximeter every 5 seconds after transferring the solution to the measuring chamber and the oxygen depletion was recorded for at least 5 minutes with the software PreSens Measurement Studio 2.
Temperature The temperature was determined in the first replicate with and without ATU at test start with the pH-meter. The room temperature
during the test was recorded continuously.
Atmospheric pressure The atmospheric pressure was determined with a barometer before the first replicate was measured (needed for measurement of the
oxygen concentration). - Reference substance (positive control):
- yes
- Remarks:
- N-Methylaniline
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 160 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 36.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 556 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of heterotrophic respiration
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 55.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of heterotrophic respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of heterotrophic respiration
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 98.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 35 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Results with reference substance (positive control):
- The 3-hour EC50 value for the reference item N- Methylaniline, was EC50 = 2.32 mg/L with 95% Confidence Interval = 1.79 – 3.03 mg/L, thus confirming the acceptability of the inoculum used in the test system.
In the reference test the EC50-value for copper (II) sulphate pentahydrate was determined by linear regression with: EC50 = 86.8 mg/L with 95% Confidence Interval = 83.9 – 89.7 mg/L.
This is in the recommended range of validity of 53 - 155 mg/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The NOEC of PU-2018-821 for total respiration is 16 mg/L.
The EC10 for total respiration is 36.8 mg/L and the EC50 is 160 mg/L.
The NOEC for heterotrophic respiration is 16 mg/L.
The EC10 for heterotrophic respiration is 55.8 mg/L and the EC50 is 556 mg/L.
The NOEC for nitrification is 16 mg/L.
The EC10 for nitrification is 35.0 mg/L and the EC50 is 98.2 mg/L
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