Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 700-936-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2013-07-01 to 2013-07-18
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 148559-52-8
- Cas Number:
- 148559-52-8
- IUPAC Name:
- 148559-52-8
- Details on test material:
- - Name of test material (as cited in study report): Reactive Golden Yellow HF-RN 1331
- Molecular formula: C17H11F2Na2O7S2 (as sodium salt) C17H13F2N7O7S2 (as free acid)
- Physical state: Solid, red to orange powder
- Analytical purity: 100 %
- Purity test date: 2012-10-08
- Lot/batch No.: Kilo 10 & 12
- Expiration date of the lot/batch: 2014-01-17
- Stability under test conditions: Not specified
- Storage condition of test material: Room temperature, protected from light, in original container; since 2013-03-01 additionally under nitrogen
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Stock solution 2.5 g/L
- Eluate: Synthetic waste water acc. to OECD 209
- Differential loading: Extended limit test to determine the NOEC: 250 - 500 - 1000 mg/L
- Controls: Triplicates at the beginning of the test + triplicates at the end of the test
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Preparation of inoculum for exposure: Non adapted activated sludge from the sewage plant at Hildesheim (Municipal sewage treatment plant of 31137 Hildesheim, Germany)
- Pretreatment: The sludge was washed twice with tap water and adjusted to a dry sludge concentration of 3.0 g/L ± 10 %. The sludge was used within 24 h after sampling.
- Dry sludge concentration: 3.12 g/L corresponding to 1.56 g suspended soldis/L in the test vessel
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Hardness:
- Not determined
- Test temperature:
- 20.6 °C
- pH:
- pH-value of the activated sludge 7.54
pH-value of the synthetic waste water 7.45 - Salinity:
- Not determined
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 500 mL Erlenmeyer flask, DIN 12380
- Aeration: Permanent
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 6
- Composition of test medium:
Test item was applied with a stock solution:
Test item concentration [mg/L]
250 500 1000
Inoculum [mL] 250
Synthetic waste water [mL] 16
Stock solution [g/L] 2.5
Stock solution [mL] 50 100 200
Demineralised water [mL] 184 134 34
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
After 3 h incubation, the oxygen concentration of each control, reference item and test item replicate was determined by means of oximeter immediately after transferring the solution to the measuring cell and the oxygen depletion was recorded for at least 3 minutes on a flat bed recorder.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study
Inhibitions were as follows:
Nominal Test pH Adjusted pH pH Inhibition
Item (test item (test item in (test item [%]
Concentration in demin. demin. water) in medium)
[mg/L] water)
1st preliminary test
10 6.12 - 7.45 2
100 5.64 - 7.34 4
1000 4.83 - 7.14 13
2nd preliminary test
1000 4.76 6.58* 7.35 16
1000 4.87 - 7.21 17
1000 4.75 - 7.32 16
- Reference substance (positive control):
- yes
- Remarks:
- Copper (II) sulphate pentahydrate p.a.
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 250 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: The test solutions were yellow to brown coloured and slightly foaming.
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: EC50: 110 mg/L - Reported statistics and error estimates:
- The NOEC was determined by calculation of statistical significance of the inhibition of respiration in comparison to the control. One Way Analysis of Variance (ANOVA) was used for NOEC calculations. When running a One Way Analysis of Variance a Normality test and an Equal Variance test were done first. P-values for both Normality and Equal Variance test are 0.05. The a-value (acceptable probability of incorrectly concluding that there is a difference) is a=0.05.
Any other information on results incl. tables
From the oxygen depletion the oxygen uptake rates were calculated in mg O2/L×h, additionally the specific respiration rates based on the dry sludge concentration were calculated in mg O2/g×h.
The inhibition of the oxygen uptake rates of the test and reference item concentrations in % was calculated in comparison with the oxygen uptake rates of the control.
Oxygen Uptake Rates, Specific
Respiration Rates and Inhibition of the Respiration
of the Control and Test Item Concentrations
|
Oxygen Uptake Rate |
Specific Respiration Rate |
|
Mean Inhibition |
||
Control |
1 |
51.2 |
32.8 |
– |
– |
|
2 |
62.8 |
40.3 |
||||
3 |
59.2 |
37.9 |
||||
4 |
58.4 |
37.4 |
||||
5 |
52.8 |
33.8 |
||||
6 |
55.6 |
35.6 |
||||
Test item |
250 |
1 |
54.8 |
3 |
-1 |
|
2 |
60.0 |
-6 |
||||
3 |
60.4 |
-7 |
||||
4 |
57.2 |
-1 |
||||
5 |
54.4 |
4 |
||||
500 |
1 |
56.0 |
1 |
13 |
||
2 |
49.6 |
13 |
||||
3 |
47.2 |
17 |
||||
4 |
46.8 |
17 |
||||
5 |
48.0 |
15 |
||||
1000 |
1 |
44.0 |
22 |
22 |
||
2 |
42.8 |
25 |
||||
3 |
42.4 |
25 |
||||
4 |
46.4 |
18 |
||||
5 |
44.0 |
22 |
Repl. = Replicate
Mean specific respiration rate of the control: 36.3 mg O2/g×h
Coefficient of variation of oxygen uptake rates of the control: 7.6 %
Suspended solids in the test vessel: 1.56 g/L
Oxygen Uptake Rates, Inhibition of the Respiration of the Reference Item Concentrations
|
Oxygen Uptake Rate |
Specific Respiration Rate |
|
Mean Inhibition |
||
Reference item |
58 |
1 |
46.0 |
19 |
17 |
|
2 |
46.8 |
17 |
||||
3 |
48.4 |
15 |
||||
100 |
1 |
32.4 |
43 |
47 |
||
2 |
29.2 |
49 |
||||
3 |
29.2 |
49 |
||||
180 |
1 |
15.2 |
73 |
74 |
||
2 |
14.0 |
75 |
||||
3 |
15.2 |
73 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The NOEC of the test item Reactive Golden Yellow HF-RN 1331 is 250 mg/L, the EC50 - value of the test item is > 1000 mg/L.
The test item is not toxic at the concentration of 250 mg/L to activated sludge of a municipal sewage treatment plant. - Executive summary:
A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 was carried out with the test item Reactive Golden Yellow HF-RN 1331 (batch number: Kilo 10&12) from 2013-07-01 to 2013-07-18, with the definitive experimental phase on 2013-07-18 at the Dr.U.Noack-Laboratorien, 31157 Sarstedt, Germany. Test system was activated sludge of the municipal treatment plant of 31137 , . The test was carried out under static conditions with the substance concentrations of 250 - 500 - 1000 mg/L. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. The mean inhibition of respiration for the test item replicates were - 1 %, 13 % and 22 %. The NOEC of the test item Reactive Golden Yellow HF-RN 1331is 250 mg/L, the EC50 - value of the test item is > 1000 mg/L.
In order to check the activity of the test system and the test conditions a reference test was carried out with copper (II) sulphate pentahydrate as reference item and the reference toxicity was determined. The EC50-value for the reference item was 110 mg/L.
NOEC and EC-Value of Acid Red RN 2949
EC-value
[mg/L]NOEC*
250
EC50
> 1000
*) No statistically significant effect (p 0.05), ANOVA, Dunnett’s Method
The NOEC of the test item Reactive Golden Yellow HF-RN 1331 is
250 mg/L.
The test item is not toxic at the concentration of 250 mg/L to activated sludge
of a municipal sewage treatment plant.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.