Benzoyl chloride, 4-[[(2-methoxybenzoyl)amino]sulfonyl]-

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

13 May - 21 Jun 2006

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Remarks:

HPLC-UV/VIS

Details on sampling:

- Concentrations: 0 and 100 mg/L
- Sampling method: Aliquots of the samples from the biological test were directly analysed by HPLC
- Sample storage conditions before analysis: Routinely, the samples are analysed immediately. Only in exceptional cases, they are stored overnight deeply frozen and protected from light.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: To produce the only test concentration 504.1 mg of the test item was added to 5 L of dilution water, then treated with an ultrasonic bath for 1 h and afterwards stirred for 24 h on a magnetic stirrer. Finally undissolved particles of the test item were removed by filtration using a folded filter of pore size 7-12 µm.
- Controls: Yes
- Test concentration separation factor: Single concentration was used
- Evidence of undissolved material: undissolved particles of the test item were removed by filtration

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish (Danio rerio)
- Source: Aqua KloGer (Germany)
- Length at study initiation: Mean standard length (n = 20) was 3.17 cm (S.D. = 0.25 cm)

ACCLIMATION
- Acclimation period: Stock held since 2006-03-03 and acclimatised to the test conditions since then till begin of test.
- Acclimation conditions: Same as test (20 - 24 °C and dissolved oxygen > 5 mg/L)
- Type and amount of food during acclimation: Commercial fish food, daily. Discontinued 24 h prior to test.
- Health during acclimation: < 5 % mortality

FEEDING DURING TEST
no feeding during the exposure period

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

14.9 °dH (= 266 mg/L CaCO3)

Test temperature:

20.3-21.4 °C over 4 days

pH:

7.6 - 7.9 over 4 days

Dissolved oxygen:

8.1 - 9.1 % over 4 days

Nominal and measured concentrations:

Nominal: 100 mg/L
Measured: 89.076 mg/L at 0 h and 91.001 mg/L at 96 h

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquaria holding 5 L of test media
- Aeration: gentle aeration via narrow glass tubes
- No. of organisms per vessel: 10
- No. of vessels per concentration: 1
- No. of vessels per control: 1
- Loading: 0.53 g body weight (wet weight) per litre

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water prepared according to the recommendations of ISO 7346.
- Culture medium different from test medium: No. This freshly prepared standard dilution water is used for both, the maintenance of the test animals under flow-through conditions and the preparation of stock and test solutions of the test item.

OTHER TEST CONDITIONS
- Adjustment of pH: Yes (pH adjusted on first day for the treatment from 5.4 to 7.9)
- Photoperiod: 16 h light: 8 h dark

EFFECT PARAMETERS MEASURED
- Mortalities are recorded at 24-hour intervals

TEST CONCENTRATIONS
- Spacing factor for test concentrations: single concentration was used

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Other abnormalities: None
- Mortality of control: 0 %

Sublethal observations / clinical signs:

Chemical analysis for control

Chemical analysis	0 h	24 h	48 h	72 h	96 h
HPLC replicates (mg/L)	<0.046 <0.046	----	----	----	<0.046 <0.046
HPLC mean value (mg/L)	<0.046	----	----	----	<0.046

 

Chemical analysis for 100 mg/L

Chemical analysis	0 h	24 h	48 h	72 h	96 h
HPLC replicates (mg/L)	89.104 89.047	----	----	----	91.095 90.906
HPLC mean value (mg/L)	89.076	----	----	----	91.001

Validity criteria for the measurement of the fish toxicity

Target condition according to guideline:	Actual condition according to the study:	Validity criteria met:
In the control(s) (dilution water control, solvent control), the mortality should not exceed 10% (or one fish, if fewer than 10 control fish are tested) at the end of the exposure	No mortality	Yes
The dissolved oxygen concentration should be ≥60% of the air saturation value in all test vessels throughout the exposure	The dissolved oxygen concentration was at least 91 %	Yes
There should be evidence that the concentration of the test substance has been satisfactorily maintained, and preferably it should be at least 80% of the nominal concentration throughout the exposure. If the deviation from the nominal concentration is >20%, results should be based on the measured concentration (geometric mean in static and semi-static tests, arithmetic mean in flow-through tests (3); time weighted average, where applicable).	All results are expressed in terms of nominal concentrations. Recovery rates correspond to 89.1 % at 0 hours and to 91 % at 96 hours respectively.	Yes
Temperature should be appropriate to the specified test species, e.g., for zebrafish within the range of 20-24°C and should not vary more than ± 1°C between test vessels and between successive days while staying in the recommended range.	Control: range = 20.3-21.4 °C over 4 days mean = 20.6 °C Frequency of measurment = once per day Test substance 100 mg/L range = 20.4-21.3 °C over 4 days mean = 20.6 °C Frequency of measurment = once per day	Yes

 

Validity criteria fulfilled:

yes

Remarks:

See more details in the table in the section "Any other information on results incl. tables"

Conclusions:

No toxic effects against fish at a concentration of 100 mg/L; 96h LC50 > 100 mg/L (nominal test concentration)

Executive summary:

In a 96-h acute toxicity study, Danio rerio were exposed to the test substance at a limit test concentration of 100 mg/L (nominal concentration) and control under static conditions. The study was conducted in accordance to the EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (O.J. No. L383A, 29.12.92) Part C, Method 1 'Acute toxicity for Fish'. No toxic effects against fish were observed at the limit concentration of 100 mg/L and therefore the LC50 is >100 mg/L. The results are expressed in terms of nominal concentrations. The test substance is an acid chloride which easily hydrolyses to the corresponding acid in aqueous media. Therefore the acid was analysed by HPLC. Recovery rates correspond to 89.1 % at 0 hours and to 91 % at 96 hours respectively.

Description of key information

In a 96-h acute toxicity study, Danio rerio were exposed to the test substance at a limit test concentration of 100 mg/L (nominal concentration) and control under static conditions. The study was conducted in accordance to the EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (O.J. No. L383A, 29.12.92) Part C, Method 1 'Acute toxicity for Fish'. No toxic effects against fish were observed at the limit concentration of 100 mg/L and therefore the LC50 is >100 mg/L. The results are expressed in terms of nominal concentrations. The test substance is an acid chloride which easily hydrolyses to the corresponding acid in aqueous media. Therefore the acid was analysed by HPLC. Recovery rates correspond to 89.1 % at 0 hours and to 91 % at 96 hours respectively.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

> 100 mg/L