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EC number: 602-890-1 | CAS number: 122731-58-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Jan 1996
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1997
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- Deviations:
- yes
- Remarks:
- In deviation from the guidelines, nutrient medium according to DIN 38412 Part 8 was not used for the preculture; instead, nutrient medium according to KIELWEIN was used.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- rac-N-(2,3-Dihydroxypropyl)-5-nitroisophthalamic acid
- EC Number:
- 602-890-1
- Cas Number:
- 122731-58-2
- Molecular formula:
- C11 H12 N2 O7
- IUPAC Name:
- rac-N-(2,3-Dihydroxypropyl)-5-nitroisophthalamic acid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- A preculture was set up in nutrient medium 1 day in advance of the actual test, according to KIELWEIN; incubation took place under test conditions; dilution to 10 ± 5 [TE/F] on the day of testing; incubation took place for another 7 ± 1 eh] under test conditions; dilution to 10 ± 5 [TE/F] was carried out with nurient medium according to KIELWEIN
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 16 h
Test conditions
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL
- Type (delete if not applicable): closed
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 16 h
- Dose descriptor:
- EC50
- Effect conc.:
- 650 mg/L
- 95% CI:
- >= 262 - <= 1 612
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Key result
- Duration:
- 16 h
- Dose descriptor:
- EC10
- Effect conc.:
- 378 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
Any other information on results incl. tables
Mean values of turbidity (expressed as TE/F) and growth inhibition of Pseudomonas putida as a function of the concentration (n:3) of NIP-Monoamide
Nominal concentration of NIP-Monoamide [mg/I] | TE/F [mean value (16 hours)] | Growth inhibition (%) |
Control | 77 | 0 |
31 | 77 | 0 |
63 | 92 | -20 |
125 | 152 | -98 |
250 | 174 | -127 |
500 | 31 | 60 |
1000 | 4 | 96 |
2000 | 3 | 97 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 for growth inhibition of Pseudomonas was calculated to be 650 mg/L [confidence limits (262 - 1612 mg/L)].
- Executive summary:
The purpose of this study was to determine the growth inhibition of Pseudomonas putida under exposure of NIP-Monoamide (ZK 39166). The test was conducted in agreement with the DIN standard 38412, L8, "Wachstumshemmtest mit dem Bakterium Pseudomonas putida", March 1991.
The test substance was incubated in an aqueous solution including nutrients with a bacterial population of Pseudomonas putida for a test duration of approximately 16 hours. The tested nominal concentrations were 31, 63, 125, 250, 500, 1000 and 2000 mg/L. Additionally, one control without the test substance was used.
The results indicate that lower concentrations of NIP-Monoamide stimulate the growth of Pseudomonas putida, while concentrations of > 500 mg/L had an inhibitory effect. The remarkable increase of growth in the concentrations of 63 to 250 mg/L could not be explained, since the nutrient medium should be sufficient for an optimal (logarithmic) growth of the controls. In any case, it can be concluded that there was no growth inhibition up to concentrations of 250 mg/L. The EC10 was calculated with 378 mg/L (no confidence limits given). The EC50 was calculated with 650 mg/L [confidence limits (262 - 1612 mg/L)].
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