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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Cross-reference
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2006
Report date:
2006

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Principles of method if other than guideline:
The following deviations from the study plan were documented:
The highest variation of the pH-value in the test exceeds that accepted by the guideline. As no significant immobility was determined, this was considered as uncritical.
The temperature for the main study was in a range of 22.0 to 23.0 °C and therefore higher than stated in the guidelines. As no immobility occurred during the test, this deviation was considered as uncritical.
The temperature for the reference study was within the range of 21.5 to 22.0 °C and therefore higher than stated in the guidelines. As the calculated EC50i was within the required range, this deviation was considered as uncritical
GLP compliance:
yes (incl. QA statement)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 110 mg/L
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
110 mg/L
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 110 mg/L
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
110 mg/L
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
24h-NOEC = 111 mg/L
48h-NOEC = 111 mg/L
24h-EC50i > 111 mg/L
48h-EC50i > 111 mg/L
Executive summary:

For the determination of 24h- and 48h-EC50i of MDA against Daphnia magna STRAUS according to OECD 202 resp. EU C.2, the main study was performed as a limit test using the nominal concentration 110 mg/L. Twenty daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted. No significant immobilisation was observed in the treatment and in the control. The 24h-EC50i of potassium dichromate was tested in a current reference test. The value was determined as 1.4 mg/L which is within the required range of 0.6 to 2.1 mg/L.

The following biological results could be determined for the test item MDA (species: Daphnia magna):

24h-NOEC = 111 mg/L

48h-NOEC = 111 mg/L

24h-EC50i > 111 mg/L

48h-EC50i > 111 mg/L