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EC number: 287-024-7 | CAS number: 85408-62-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994-08-04 to 1994-08-04
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Short summary report of well conducted GLP study performed to OECD guideline 209
Data source
Reference
- Reference Type:
- other: short summary report incl. all critical study information e.g. OECD guideline, test item identity, result, study dates, etc.
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
- Remarks:
- Test carried out to principles of GLP; but, the testing was carried out without inspection by the QA unit.The report was prepared in simplified summary form and contains no GLP certificate. Details of test material summarised in report but CofA not avail
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Details on test material:
- Batch: 1 (7.93) 331302
Purity: 68.7%
Impurities: 25% (coconut fatty acid and stearic acid)
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: in the range of 62.5mg/L and 1000mg/L
- Sampling method: no data
- Sample storage conditions before analysis: no data
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Due to low solubility, there was no stock solution (no solvent). The test item was weighed directly into the vessels. Better solubility was achieved in the test vessels by ultrasound treatment 30 minutes before the beginning of the test.
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- Amount of activated sludge used: 1600mg/L (dry weight)
Origin: Municipal wastewater treatment plant, Frankfurt/M-Sindlingen
Preparation: As specified in cited test guidelines
Acclimation state: Not acclimatised to the test substance or reference substance in the lab.
Study design
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- no data
Test conditions
- Hardness:
- no data
- Test temperature:
- 20 +/- 2 degrees C
- pH:
- 7.2 +/- 0.2
- Dissolved oxygen:
- no data
- Salinity:
- not applicable
- Nominal and measured concentrations:
- The concentrations of the test item were checked to be in the range of 62.5mg/L to 1000mg/L.
- Details on test conditions:
- The incubation with the test item was performed before the measurement of respiratory activity at 20 +/- 2°C over a period of 3 hours. The composition of the reaction medium is equivalent to that specified in the cited EEC Directive. The pH value of the culture solution was 7.2 +/- 0.2 and was determined prior to the depletion measurements. The sensitivity of the activated sludge was tested with a reference approach. A reference substance of 3,5-Dichlorphenol at the concentrations 5, 20 & 30 mg/L. As a control, an inoculated approach was used without addition of a test material. The control was filled with water to achieve a uniform volume.
The specified testing ranges were kept with both the reference substance as well as the control. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorphenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- ca. 639 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no data
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- The specified testing ranges were kept with both the reference substance as well as the control.
- Results with reference substance valid? no data
- Relevant effect levels: no data - Reported statistics and error estimates:
- No data
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test item was found to be harmless to the organisms in activated sludge.
The EC50 is in the range: >1000mg/L - Executive summary:
In a OECD 209 Activated sludge, respiration inhibition test, the inoculum (mainly domestic activated sludge) was exposed to the test item for 3 hours. Due to low solubility, there was no stock solution. The test item was weighed directly into the vessels. Better solubility was achieved in the test vessels by ultrasound treatment 30 minutes before the beginning of the test. The EC50 of the test item was found to be >1000mg/L, which means the test item is harmless to the organisms in activated sludge.
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