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EC number: 276-743-1 | CAS number: 72624-02-3
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
Exposure of Scenedesmus subspicatus to the test material, based on nominal test concentrations, gave an EbC50 (72h) value of 0.25 mg/L; 95% confidence limits 0.21 - 3.0 mg/L and an ErC50 (0 -72h) value of 1.2 mg/L (It was not possible to calculate 95% confidence limits for the ErC50 value as the data generated did not fit the models available for calculation of confidence limits.) The NOEC (72h) was 0.048 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 1.2 mg/L
Additional information
In a key study performed to OECD Guideline No 201 and EC Method C3, following a preliminary range-finding test, Scenedesmus subspicatus was exposed to solutions of the test material at nominal concentrations of 0.048, 0.095, 0.19, 0.38, 0.75 and 1.5 mg/L (three replicate flasks per concentration) for 72 hours, under constant illumination and shaking at a temperature of 24±1°C. The test material solutions were prepared by shaking an excess (100 mg/L) of test material in culture medium at 300 rpm at a temperature of 30°C for 24 hours prior to removal of any undissolved test material by filtration (0.2µm) through a preconditioned filter to produce a saturated solution with a nominal test concentration of 30 mg/L. This "saturated" solution was then further diluted, as necessary, to provide the remaining test groups. The nominal test concentrations were based on the results of chemical analysis of a saturated solution prepared during the range-finding test where the measured concentration of the saturated solution was 31.7 mg/L.
Samples of the algal populations were removed daily and cell concentrations determined for each control and treatment group, using a Coulter Multisizer Particle Counter.
Exposure of Scenedesmus subspicatus to the test material, based on nominal test concentrations, gave an EbC50 (72h) value of 0.25 mg/L; 95% confidence limits 0.21 - 3.0 mg/L and an ErC50 (0 -72h) value of 1.2 mg/L (It was not possible to calculate 95% confidence limits for the ErC50 value as the data generated did not fit the models available for calculation of confidence limits.) The NOEC (72h) was 0.048 mg/L.
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