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EC number: 271-351-7 | CAS number: 68541-71-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- - Preparation of the inoculum : higher sludge weight after the washing procedure, - 4 instead of 5 test concentrations and - T° 22.4 °C instead of 20+/-2 °C
- Qualifier:
- according to guideline
- Guideline:
- other: EEC Method 87/302, L 133/118 -122 "Activated Sludge, Respiration Inhibition Test", (Edition : 30.05.1988)
- Deviations:
- yes
- Remarks:
- - Preparation of the inoculum : higher sludge weight after the washing procedure, - 4 instead of 5 test concentrations and - T° 22.4 °C instead of 20+/-2 °C
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Code number: FAT-20011/D
Batch-Nr: 61
Purity: ca 52.5 %
Appearance: solid
Solubility: miscible
Storage: room temperature
Expiration date: 07/99 - Analytical monitoring:
- no
- Vehicle:
- yes
- Remarks:
- deionized water
- Details on test solutions:
- Stock solutions: 500 mg/l test substance / reference substance in deionized water.
- Test organisms (species):
- other: Activated sludge of a communal sewage treatment plant (ARA-Pro Rheno Basel)
- Details on inoculum:
- - Pretreatment: 3 times washing with tap water and addition of synthetic sewage (a day before the main test).
- Sludge dry weight: 1.50 g/l - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 22.4 °C
- pH:
- 7.3 before the test beginning.
- Nominal and measured concentrations:
- Test concnetrations (nominal concentrations)
- 320,100, 32 and 10 mg/l of the test substance.
- 320, 10 and 10 mg/l for the reference substance. - Details on test conditions:
- Experimental conditions
- Sludge dry weight: 1.50 g/l
- Test volume: 100 ml
- Test duration: 3 hours
- Estimation technique: O2 - consumption, measured with an oxygen sensitive electrode system : OXI DIGI 530 (SAV : 00 109)
- Aeration: 0.1 - 0.2 l/min by pasteur-pipette
- pH of sludge: 7.3 before the test beginning - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol (Merck 821768)
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 320 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: IC20
- Effect conc.:
- 110 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- IC50 (3 h) of the reference substance 3,5-Dichlorophenol: 12 mg/l
- Validity criteria fulfilled:
- yes
- Conclusions:
- The IC50 (3 h) of FAT 20011/D was >320 mg/l
- Executive summary:
The toxicity of FAT 20011/D to activated sludge was determined in a 3-hour respiration inhibition test according to the EEC Method No. L 133/118 -122 and to the OECD Guideline No. 209. The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations of Switzerland.
The test substance concentrations tested were 320,100, 32 and 10 mg/L. The reference substance, 3,5-Dichlorophenol was tested in concentrations of 32,10 and 3.2 mg/L.
Results:
IC50 (3 h) of the test substance FAT 20011/D: >320 mg/L
IC20 (3 h) of the test substance FAT 20011/D: 110 mg/L
IC50 (3 h) of the reference substance 3,5-Dichlorophenol: 12 mg/L
Reference
Description of key information
The toxicity of FAT 20011/D to activated sludge was determined in a 3-hour respiration inhibition test conducted according to the EEC Method No. L 133/118 -122 and the OECD Guideline 209, in compliance with GLP. The test substance concentrations tested were 320,100, 32 and 10 mg/L. Based on the findings of the study, the IC50 of FAT 20011/D was determined to be >320 mg/L.
The above outcome was further supported by the results of supporting studies conducted in 1982 (FAT 20011/C) and 1976 (FAT 20011/B), where IC50s were reported as 100 and >320 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 320 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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