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Diss Factsheets
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EC number: 244-691-9 | CAS number: 21981-33-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study in accordance to OECD GL
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 1,1,1,3,3-pentachlorobutane
- EC Number:
- 244-691-9
- EC Name:
- 1,1,1,3,3-pentachlorobutane
- Cas Number:
- 21981-33-9
- Molecular formula:
- C4H5Cl5
- IUPAC Name:
- 1,1,1,3,3-pentachlorobutane
- Details on test material:
- - Name of test material (as cited in study report): pentachlorobutane
- Molecular formula (if other than submission substance): same as submission substance
- Molecular weight (if other than submission substance): same as submission substance
- Smiles notation (if other than submission substance): same as submission substance
- Structural formula attached as image file (if other than submission substance): see Fig.
- Substance type: organic
- Physical state: liquid
- Analytical purity: 90%
- Impurities (identity and concentrations):
- Purity test date: 09/09/99
- Lot/batch No.: 99350L08
- Expiration date of the lot/batch: 13/09/2000
- Stability under test conditions: stable
- Storage condition of test material: at room temeprature in the dark
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Stock solution was prepared by adding 36 mg test substance to 0.5 g Chromosorb G (60-80 mesh) in a headspace vial to get a homogeneous solution in water. The vial was placed on a roller bank in a climate chamber at 20°C for 1 hour. Afterward, the content was transferred into a 320 ml flask completely filled with Elendt medium and closed with a screw cap and stored at room temperature for 3 days. The stock solution concentration was determined on the day of the test initiation to be 41.4 mg/l. The test solutions were prepared by consecutive dilutions.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water fleas
- Strain: Daphnia magna
- Source: Daphtoxkit F. magna (Bio International , the Netherlands)
- Age at study initiation (mean and range, SD): 0-24 h
- Weight at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): not reported
- Method of breeding: cultured in Elendt M4 medium in a climate chamber (20°C). 16 h light/day.
- Feeding during test
- Food type: Chlorella vulgaris
- Amount and frequency not stated
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- Standard Elent M4 medium
- Test temperature:
- 20.4-21.2
- pH:
- 7.9 at day 0, 7.5-7.6 at day 2
- Dissolved oxygen:
- 8.4 mg/l at day 0, 8.5-8.6 at day 2
- Salinity:
- Standard Elent M4 medium
- Nominal and measured concentrations:
- - nominal concentrations: 0, 0.2, 0.4, 0.8, 1.6 and 3.2 mg/l
- measured concentrations (mean value): 0 (n.d.), 0.07, 0.26, 0.58, 1.3, 2.4 mg/l - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: Glass, no headspace, 320 ml
- Aeration: No
- Type of flow-through (e.g. peristaltic or proportional diluter): n/a
- Renewal rate of test solution (frequency/flow rate): n/a
- No. of organisms per vessel: 10-12
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ultrapure water prepared by reversed osmosis, followed by anion, cation and mixed bed chromatography and filtered through 0.22 um cellulose filter
- Conductivity: < 4 uS/cm
- Culture medium different from test medium: No - Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- ca. 1.8 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidence interval: 1.3-2.4 mg/l
- Details on results:
- - Behavioural abnormalities: 2 organisms lying on the bottom of the flask were observed at the NOEC (1.3 mg/l)
- Observations on body length and weight: n/a
- Other biological observations: no
- Mortality of control: No mortality or immobility observed
- Other adverse effects control: No
- Abnormal responses: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
Any other information on results incl. tables
No immobility was observed in the control samples and up to 1.3 mg/l (measured conc.). 100% immobility was observed in the 3 samples at 2.4 mg/l (measured concentrations). A 48 hours EC50 was calculated by the geometric mean of the highest concentration without effect and the lowest concentrations with 100% immobility leading to a 48 -h EC50 of 1.8 mg/l.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The substance is classifiable as toxic to Daphnia
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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