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EC number: 235-468-7 | CAS number: 12237-62-6 This substance is identified in the Colour Index by Colour Index Constitution Number, C.I. 42535:3.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Data is from experimental study report
- Qualifier:
- according to guideline
- Guideline:
- other: EEC, L 251 Vol. 27 (19.09.84) 84/449/EEC,C.5
- Deviations:
- yes
- Remarks:
- The volume of the test solution was reduced from 3.01 to 1.51.
- Principles of method if other than guideline:
- The CO2 formed by biodegradation was absorbed with NaOH and determined on a carbon analyzer. Due to the poor solubility of the test substance in water, an emulsifier was used to achieve a better distribution in the medium. The test substance was added to the medium, homogenized with Nonylphenol 10EO5PO. Instead of 10 and 20 mg/L, 10 and 30 mg/L were tested.
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: Bacteria collected from a sewage treatment plant.
- Details on inoculum:
- Bacteria collected from activated sludge of the sewage treatment plant of CH-4106 Therwil on 16/10/89 The preparation was carried out according to the method described in the guideline.
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 10.9 mg/L
- Based on:
- test mat.
- Initial conc.:
- 31.4 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- Preparation of test material:
1200 ml of the mineral solution with the inoculum were aerated for 24 hours in the test vessel. In 300 ml mineral solution 0.5 ml Nonylphenol 10EO5PO (solution of 30 mg in 100 ml bidist. water) and 16.3 rsp. 47.1 mg of test substance were added and homogenized. This solution was given to the test vessel which was immediately connected to the CO2 traps.
Vessels : 2 Liter flasks (dark brown glass) equipped with gas inlet and magnetic stirrer.
Water : The test medium was prepared according to the method described in the guideline
Duration : 28 days
Air : = 25 ml/min purified from carbon dioxide. - Reference substance:
- aniline
- Remarks:
- MERCK No. : 1261, Batch : 12/09/89 Stability : 12/03/90 Storage : room temperature; Reference substance : 20 mg/L
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 6
- Sampling time:
- 28 d
- Remarks on result:
- other: 10.9 mg test substance/l
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 4
- Sampling time:
- 28 d
- Remarks on result:
- other: 31.4 mg test substance/l
- Details on results:
- Theoretical carbon dioxide evolution
Test substance : 10.9 mg/L = 29.83 mg COa/l.S liter,
Test substance : 31.4 mg/L = 86.20 mg COa/l.S liter,
This calculation is based on carbon analysis.
Biodegradation
Test substance : The biodegradation was calculated as :
10.9 mg test substance/l = 6% in 28 days.
31.4 mg test substance/l = 4% in 28 days. - Validity criteria fulfilled:
- not specified
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The percentage degradation of test substance was determined to be 6.0 and 4.0% (using 10.9 and 30.4 mg/l conc. of test substance) by using CO2 evolution parameter in 28 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.
- Executive summary:
28-days biodegradation test following the EU Method C.5 (Degradation: Biochemical Oxygen Demand) was performed to determine the ready biodegradability of the test chemical . Bacteria was used as a test inoculum for the study. Bacteria was collected from activated sludge of the sewage treatment plant of CH-4106 Therwil on 16/10/89 The preparation was carried out according to the method described in the guideline. Aniline (20 mg//l) was used as a reference substance for the study. 1200 ml of the mineral solution with the inoculum were aerated for 24 hours in the test vessel. In 300 ml mineral solution 0.5 ml Nonylphenol 10EO5PO (solution of 30 mg in 100 ml bidist. water) and 16.3 rsp. 47.1 mg of test substance were added and homogenized. This solution was given to the test vessel which was immediately connected to the CO2 traps. The CO2 formed by biodegradation was absorbed with NaOH and determined on a carbon analyzer. Due to the poor solubility of the test substance in water, an emulsifier was used to achieve a better distribution in the medium. Determination of the initial CO2 of the 0.05 N sodium hydroxide and the CO2, absorbed in the absorbers filled with 200 ml 0.05 N sodium hydroxide was carried out on the days 3, 7, 10, 13, 17, 20, 24, 27 and 28, respectively. The biodegradation was calculated on the basis of the theoretical carbon content of the test substance and the cumulative quantities of carbon dioxide determined on the days of measurements. The percentage degradation of test substance was determined to be 6.0 and 4.0% (using 10.9 and 30.4 mg/l conc. of test substance) by using CO2 evolution parameter in 28 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.
Reference
Description of key information
28-days biodegradation test following the EU Method C.5 (Degradation: Biochemical Oxygen Demand) was performed to determine the ready biodegradability of the test chemical . Bacteria was used as a test inoculum for the study. Bacteria was collected from activated sludge of the sewage treatment plant of CH-4106 Therwil on 16/10/89 The preparation was carried out according to the method described in the guideline. Aniline (20 mg//l) was used as a reference substance for the study. 1200 ml of the mineral solution with the inoculum were aerated for 24 hours in the test vessel. In 300 ml mineral solution 0.5 ml Nonylphenol 10EO5PO (solution of 30 mg in 100 ml bidist. water) and 16.3 rsp. 47.1 mg of test substance were added and homogenized. This solution was given to the test vessel which was immediately connected to the CO2 traps. The CO2 formed by biodegradation was absorbed with NaOH and determined on a carbon analyzer. Due to the poor solubility of the test substance in water, an emulsifier was used to achieve a better distribution in the medium. Determination of the initial CO2 of the 0.05 N sodium hydroxide and the CO2, absorbed in the absorbers filled with 200 ml 0.05 N sodium hydroxide was carried out on the days 3, 7, 10, 13, 17, 20, 24, 27 and 28, respectively. The biodegradation was calculated on the basis of the theoretical carbon content of the test substance and the cumulative quantities of carbon dioxide determined on the days of measurements. The percentage degradation of test substance was determined to be 6.0 and 4.0% (using 10.9 and 30.4 mg/l conc. of test substance) by using CO2 evolution parameter in 28 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
28-days biodegradation test following the EU Method C.5 (Degradation: Biochemical Oxygen Demand) was performed to determine the ready biodegradability of the test chemical . Bacteria was used as a test inoculum for the study. Bacteria was collected from activated sludge of the sewage treatment plant of CH-4106 Therwil on 16/10/89 The preparation was carried out according to the method described in the guideline. Aniline (20 mg//l) was used as a reference substance for the study. 1200 ml of the mineral solution with the inoculum were aerated for 24 hours in the test vessel. In 300 ml mineral solution 0.5 ml Nonylphenol 10EO5PO (solution of 30 mg in 100 ml bidist. water) and 16.3 rsp. 47.1 mg of test substance were added and homogenized. This solution was given to the test vessel which was immediately connected to the CO2 traps. The CO2 formed by biodegradation was absorbed with NaOH and determined on a carbon analyzer. Due to the poor solubility of the test substance in water, an emulsifier was used to achieve a better distribution in the medium. Determination of the initial CO2 of the 0.05 N sodium hydroxide and the CO2, absorbed in the absorbers filled with 200 ml 0.05 N sodium hydroxide was carried out on the days 3, 7, 10, 13, 17, 20, 24, 27 and 28, respectively. The biodegradation was calculated on the basis of the theoretical carbon content of the test substance and the cumulative quantities of carbon dioxide determined on the days of measurements. The percentage degradation of test substance was determined to be 6.0 and 4.0% (using 10.9 and 30.4 mg/l conc. of test substance) by using CO2 evolution parameter in 28 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.
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