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EC number: 231-403-1 | CAS number: 7534-94-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- OECD 203, GLP, analytical monitoring.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on test solutions:
- Dilution water: purified drinking water
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Danio rerio (Teleostei, Cyprinidae)
- Strain: Danio rerio (Teleostei, Cyprinidae) (HAMILTON-BUCHANAN)
- Source: West Aquarium GmbH, D-37431 Bad Lauterberg, Germany
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): 2.3 -2.8 cm
- Weight at study initiation (mean and range, SD): mean weight: 0.25 ± 0.2 g
- Method of breeding: Laboratory bred
- Feeding during test: fish were not fed during the test (beginning 24 h prior to initiation)
- Food type: TetraMin® Hauptfutter
- Amount: no data
- Frequency: daily until 24 h before the test
ACCLIMATION
- Acclimation period: no data
- Acclimation conditions (same as test or not): in dilution water of the same quality as used for the test
- Health during acclimation (any mortality observed): no data - Test type:
- semi-static
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- varied between 21.8 and 22.1 °C
- pH:
- varied between 7.8 and 8.1
- Dissolved oxygen:
- in all test vessels between 86% and 100%
- Nominal and measured concentrations:
- 0.74, 1.32, 2.38, 4.29 and 7.72 mg/L (nominal concentrations)
0.59, 0.97, 1.81, 3.28 and 4.45 mg/ (mean measured test concentrations)
The measured values deviated by more than 20 % from nominal values at all dose levels. - Details on test conditions:
- Dilution water was prepared according to the test guideline (OECD 203) following internal SOP’s.
Test concentrations:
Aliquots of the stock solution were shaken for 24 h, then they were diluted with purified drinking water (dilution water).
Test priciples and procedure:
10 fish were used for each test concentration including controls.
Only fish in good health and free from any apparent malformation were used.
15-l glass aquaria were used as test vessels and filled with 10 l of test solution. The test animals were added to the previously prepared test solution; controls were kept in dilution water (blank).
The aquaria were slightly aerated via a glass capillary throughout the test.
The fish were exposed to various concentrations of the test substance for a period of 96 hours under semi static conditions. The test medium was changed completely every day. Visible abnormalities observed during the first 6 hours of the experiment were recorded. Mortalities and visual abnormalities were recorded at 24 h, 48 h, 72 h, and 96 h.
The test temperature was 21.8° C to 22.1 ° C. The pH of the dilution water was 7.8 to 8.1. The oxygen saturation in all test vessels was between 86 % and 100 %.
The light/dark rhythm was 14h/10h.
The pH, temperature and the oxygen concentrations of the control and of all the test concentrations were measured daily. - Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1.79 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 1.58 - 2.04 (95% confidence limits of LC50)
- Duration:
- 96 h
- Dose descriptor:
- LC10
- Effect conc.:
- 1.45 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.97 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: The effect concentrations refer to the mean measured concentrations, because the measured concentrations deviated by more than 20 % from the nominal values.
- Duration:
- 96 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 1.81 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: The effect concentrations refer to the mean measured concentrations, because the measured concentrations deviated by more than 20 % from the nominal values.
- Details on results:
- The measured values deviated by more than 20 % from nominal values at all dose levels. Thus, the mean measured concentrations were taken as basis for the calculation of the lethal effect concentrations.
- Sublethal observations / clinical signs:
Test concentrations - nominal and measured concentrations of the test substance Measured test concentrations Nominal test concentrations [mg/L] 0h 24h* 48h 72h 96h Mean ± s.d. Deviations [%] from nominal values control 0.00 0.00/0.00 0.00 0.00 0.00 0.00 ± 0.00 0.74 0.58 0.44/0.66 0.65 0.67 0.55 0.59 ± 0.09 - 20.2 1.32 0.89 0.67/1.11 1.11 1.16 0.91 0.97 ± 0.18 - 26.4 2.38 1.58 1.19/2.39 1.91 2.04 1.76 1.81 ± 0.39 -24.0 4.29 2.97 2.28/3.93 3.92(old) n.d. n.d. 3.28 ± 0.74 -23.7 7.72 4.71 4.19/n.d. n.d. n.d. n.d. 4.45 ± 0.30 -42.4 (*) test concentration before renewal of the test solution/ freshly prepared test solution;
s.d. = standard deviation;
n.d. = not determined (all fish dead)
Cumulative Mortality of Danio rerio (Zebra Fish) (a) Cumulative mortality of fish Nr Test concentration (mg/l) 24h 48h 72h 96h 0/0 blanc 0 0 0 0 1 0.59 0 0 0 0 2 0.97 0 0 0 0 3 1.81 0 0 4 5 4 3.28 1 10 10 10 5 4.45 10 10 10 10 (a) In all test concentrations the fish were inconspicuous during the exposure time of 96h
- Validity criteria fulfilled:
- yes
- Remarks:
- -Criteria of OECD 203: Mortality of controls <1
- Conclusions:
- In a valid guideline study (OECD 203, semi-static conditions), the 96 hr LC50 for Danio rerio (Teleostei, Cyprinidae) is 1.79 mg/L .
- Executive summary:
In a 96-h acute toxicity study, Danio rerio (Teleostei, Cyprinidae) were exposed to Isobornyl methacrylate
at 0 (control), 0.74, 1.32, 2.38, 4.29, 7.72 mg/L nominal and 0 (control), 0.59, 0.97, 1.81, 3.28, 4.45 mg/L measured concentrations under semi-static conditions. The 96-h LC50was 1.79 (1.58 - 2.04 95 % confidence limits) mg a.i./L. The LC10and NOEC values, based on mortality effects, were 1.45 mg/L and 0.97 mg a.i./L, respectively. The effect concentrations refer to the mean measured concentrations, because the measured concentrations deviated by more than 20 % from the nominal values. Based on the results of this study, Isobornyl methacrylate would be classified as toxic to Danio rerio (fish) in accordance with the classification system of the EU.This toxicity study is classified as acceptable and satisfies the guideline requirement for OECD 203, Danio rerio (Zebra fish) toxicity study.
Results Synopsis
Test organism size/age: mean weight: 0.25 ± 0.2 g, length: 2.3 -2.8 cm
Test Type: semi-static, change of test medium: completely every 24 hours
LC50: 1.79 mg a.i./L 95% C.I.: 1.58 to 2.04 mg a.i./L
NOEL: 0.97 mg a.i./L
LC10: 1.45 mg a.i./L}
Endpoint(s) Effected: mortality
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Reference
Description of key information
One reliable study for Isobornyl methacrylate on freshwater fish acc. OECD 203
is available. LC50 (96h) on Danio rerio was determined to be 1.79 mg/L
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 1.79 mg/L
Additional information
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