Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 227-645-2 | CAS number: 5921-65-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016-02-24 to 2016-03-14
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Analytical samples were taken from all test item concentrations and controls at test start and after 48 hours from aged solutions.
For each sampling also a retain sample was taken.
100 mL samples were taken in 250 mL HDPE bottles without stabilisation.
All samples were stored deep frozen until they were transferred to the analytical laboratory. - Vehicle:
- yes
- Details on test solutions:
- Based on the results of a non GLP range-finding test, the following nominal concentrations were tested in the main test: 4.50, 2.25, 1.13, 0.563 and 0.281 mg/L, solvent control and control.
The necessary amount of test item for preparing the stock solution S1 was weighed on a weighing scoop and transferred to a volumetric flask.
Dimethylformamide was added up to the bench mark and the solution was homogenised by shaking and treated with 10 minutes of ultrasonification.
Lower test solutions were prepared by dilution of the stock solution with DMF.
Final test solution contains 0.1 mL DMF/L - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water Flea
- Strain: Daphnia magna Straus, Clone V
- Source: Eurofins Agroscience Services EcoChem GmbH
- Age at study initiation: max. 24 hours old
- Method of breeding: single culture (1 daphnid per 100 mL) in Elendt M4 medium
- Type and amount of food: single cell green algae (Desmodesmus subspicatus)
- Feeding frequency: at least three times a week
- Feeding during test: none
ACCLIMATION
- Acclimation period: not applicable, freshly hatched daphnids - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 12°dH (214 mg/L CaCO3)
- Test temperature:
- 19.1 ± 0.1 °C
- pH:
- 7.74 ± 0.03
- Dissolved oxygen:
- 9.0 ± 0.1 mg/L
- Salinity:
- Elendt M4
- Nominal and measured concentrations:
- The analytical verification of test item concentrations in fish test medium was done by analysing the content of TOC (total organic carbon) in the samples during the test.
Every effort was made to develop an analytical method of the test item. Caprinoguanamine can be determined with isocratic HPLC and detected at
210 nm. But because of the low water solubility and UV-absorption of the test item the determination is not possible in aquatic solutions < 10 mg/L.
Because of missing chromophores Caprinoguanamine shows a too low UV absorption. Therefore, a qualitative detection is not possible at low
concentrations.
Since the stock solution was prepared in DMF the blank value of the solvent control was subtracted from the measured value for the calculation of the content of test item in the test solutions. This resulted partly in negative values, hence no reliable concentration values could be estimated. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 ml glas vessel, covered with a glass plate
- Material, size, headspace, fill volume: 50 ml
- Aeration: none
- Test procedure: static
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 5
- No. of vessels per vehicle control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 medium
OTHER TEST CONDITIONS
- Photoperiod: 16 hours photoperiod /8 hours darkness daily
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Justification for using less concentrations than requested by guideline: max. 2.2 requested by the guideline
- Range finding study
- Test concentrations: 4.50, 0.45, 0.045 mg/L
- Results used to determine the conditions for the definitive study:
After 48 hours of exposure no immobilisation was observed in the control, solvent control and up to and including 0.45 mg/L. 100 % of immobilisation was observed at the highest test itemconcentration of 4.50 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.18 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95 % confidence limit 2.47 - 3.87 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.13 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- After 24 hours of exposure no immobilisation was observed in the control, solvent control and up to and including 1.13 mg/L. 35 % immobilisation was observed in the test item concentration of 2.25 mg/L and 75 % of immobilisation was observed at the highest test item concentration of 4.50 mg/L.
After 48 hours of exposure no immobilisation was observed in the control, solvent control and up to and including 1.13 mg/L. 60 % immobilisation was observed in the test item concentration of 2.25 mg/L and 100 % of immobilisation was observed at the highest test item concentration of 4.50 mg/L. - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Mortality: 18 of 20 at 2.0 mg/L after 48 h
- EC50 48h: < 2.0 mg/l - Reported statistics and error estimates:
- Probit analysis using linear max. likelihood regression.
- Validity criteria fulfilled:
- yes
- Conclusions:
- According to the results of the test, the EC50 (48 h) for immobilisation was determined to be 2.18 mg/L (nominal).
The corresponding NOEC (48 h) was 1.13 mg/L (nominal). - Executive summary:
The 48 hour acute toxicity of Caprinoguanamine to Daphnia magna Straus was studied under static conditions. Daphnids were exposed to control, solvent control, and test chemical at nominal concentration of 0.281, 0.563, 1.13, 2.25 and 4.50 mg/L for 48 hours. Mortality/immobilization were observed at 24 and 48 hours.
The 48 – hour EC50 was 2.18 mg/L (nominal). The 48 – hour NOEC based on immobilization was 1.13 mg/L (nominal).
This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.
Results Synopsis
Test Organism Age: Daphnia magna Straus > 24 hours
Test Type: Static
EC50: 2.18 mg/L (nominal) 95% C.I.: 2.47 – 3.87 mg/L
Endpoint(s) Effected: immobilisation
Reference
Description of key information
The EC50 (48 h) for Caprinoguanamine in Daphnia magna Straus was determined to be 2.18 mg/L test item (nominal).
The corresponding NOEC (immobilization) (48 h) was 1.13 mg/L test item (nominal).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 2.18 mg/L
Additional information
The 48 hour acute toxicity of Caprinoguanamine to Daphnia magna Straus was studied under static conditions. Daphnids were exposed to control, solvent control, and test chemical at nominal concentration of 0.281, 0.563, 1.13, 2.25 and 4.50 mg/L for 48 hours. Mortality/immobilization were observed at 24 and 48 hours.
The 48 – hour EC50 was 2.18 mg/L (nominal). The 48 – hour NOEC based on immobilization was 1.13 mg/L (nominal).
This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.