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EC number: 226-430-0 | CAS number: 5398-29-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study. Activated sludge originated from a laboratory scale sewage plant treating municipal sewage.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: activated sludge from laboratory scale sewage plant treating domestic sewage
- Preparation of inoculum for exposure: The suitable aliquot of the activated sludge suspension was sieved by a finely woven mesh with a mesh size about 0.45 µm.
- Concentration of sludge: 30 mg/L dry weight - Duration of test (contact time):
- 28 d
- Initial conc.:
- 64.1 mg/L
- Based on:
- test mat.
- Initial conc.:
- 20 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to guideline
- Additional substrate: an inorganic nutrient salt medium was added.
- Solubilising agent: No solubilising agent was used.
- Test temperature: 20 - 25 °C
TEST SYSTEM
- Culturing apparatus: The DOC Die Away test was performed in flasks according to the guideline.
- Number of culture flasks/concentration: 2
- Number of blank control flasks: 2
- Number of inhibition control flasks: 1
- Number of positive control flaks: 1
- Number of adsorption control flasks: 1
- Measuring equipment: Samples for the DOC-analysis were performed as repeat determination, using a TOC-analyzer equipped with an auto sampler (Shimadzu TOC-500).
SAMPLING
- Sampling method: For preparation of the test assays, first the required volume of demineralized water and the solutions of mineral medium were dosed to the test vessels. For addition of the test- and reference substance aqueous stock solutions with suitable concentrations were prepared.
The content of DOC of the reference substance was calculated as theoretical organic carbon value (ThOC) and also used for the calculation of the required aliquot. The test assay of adsorption control (AC) was prepared in the same way. Aliquots of the prepared inoculum suspension were added to all test vessels (exceptional PC-assay): to adjust a concentration of activated sludge of 30 mg/L dry weight. The sampling in the AC test assay was performed before adding inoculum.
- Sampling frequency: daily.
- Sterility check if applicable: For avoidance of bacterial grow the assay was inhibited by adding of 1 mL mercury chloride solution on day 14 in the test medium.
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2
- Abiotic sterile control: 1
- Toxicity control: 1
- Other: Toxicity control: 1 - Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (DOC removal)
- Value:
- < 10
- Sampling time:
- 28 d
- Details on results:
- The test substance did not show inhibition of microorganisms in a toxicity control. No elimination of the test substance was observed in the treatments. thus, the test substance is considered to be neither readily nor inherently biodegradable.
- Results with reference substance:
- Total elimination was observed at day 7.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
Reference
Description of key information
Not readily/inherently biodegradable: < 10% in 28 days (OECD 301A)
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
One study investigating the ready biodegradability of 3-(amidinothio)propionic acid (CAS 5398-29-8) is available. The study was performed according to GLP and OECD guideline 301A (BASF 1995). Non-adapted activated sludge from a laboratory scale sewage plant treating domestic sewage was used as inoculum. DOC removal was measured over a test period of 28 days. No DOC removal was observed throughout the test period. Thus, the test substance is considered to be neither readily nor inherently biodegradable according to OECD criteria.
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