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EC number: 215-675-9 | CAS number: 1341-38-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Isooctyl palmitate
- EC Number:
- 215-675-9
- EC Name:
- Isooctyl palmitate
- Cas Number:
- 1341-38-4
- Molecular formula:
- C24H48O2
- IUPAC Name:
- 6-methylheptyl hexadecanoate
- Test material form:
- liquid
- Remarks:
- a colourless liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- The stock solution used in the range-finding test was prepared by dissolving 100.4 mg of the test item in 40 mL of dilution water – M4 and the total volume was completed to 1000 mL, resulting in a concentration of 100 mg.L-1. To assist solubilization, the solution was stirred for 20 minutes in a magnetic stirrer and 30 minutes of ultrasonic dispersion was also applied.
The stock solution used in the definitive test was prepared by dissolving 10.3 mg of the test item in 40 mL of dilution water – M4 and the total volume was completed to 1000 mL, resulting in a concentration of 10 mg.L-1. To assist solubilization, the solution was stirred for 30 minutes in a magnetic stirrer and 35 minutes of ultrasonic dispersion was also applied.
The test solutions were prepared from dilutions of the stock solutions. The preparation procedure is described in the raw data and in Tables 1 and 2.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The test system used for this study is described below, according to Straus (1820):
Kingdom: Animalia
Phylum: Arthropoda
Subphylum: Crustacea
Class: Branchiopoda
Order: Cladocera
Family: Daphniidae
Genus: Daphnia
Species: Daphnia magna
Young Daphnia magna ≤ 24 hours old were obtained from the culture kept at the Ecotoxicology Laboratory, maintenance and cultivation records are archived at BIOAGRI.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 236.2 mg.L-1 CaCO3
- Test temperature:
- 20.4°C
- pH:
- 7.7 to 7.8
- Dissolved oxygen:
- 8.1 mg.L-1
- Nominal and measured concentrations:
- Nominal and Effective Concentrations of Test Item, and Their Respective Deviations, Dilutions Factor and Chromatograms Results.
Nominal concentrations
(mg.L-1) Concentrations found in the chromatograms
(µg.L-1) Dilutions
factor Effective concentrations (mg.L-1) Average effective concentrations
(mg.L-1) Deviations
(%)
0 48 0 48 0 48
(Hours) (Hours) (Hours)
2 405.59 373.82 4.0000 4.3478 1.6412 1.6442 1.6427 17.86
1 428.28 311.81 2.0000 2.5641 0.8665 0.8088 0.8377 16.23
0.5 195.33 183.23 2.0833 2.2727 0.4117 0.4213 0.4165 16.71
0.25 109.11 84.91 2.0000 2.3810 0.2208 0.2045 0.2126 14.94
0.125 113.65 104.57 1.2500 1.2500 0.1437 0.1322 0.1380 10.38
Control < PLQ < PLQ ---- ---- ---- < PLQ < PLQ
The effective concentrations were determined according to the equations (1 and 2) described in the item 5.8.1 and exemplified in the appendix 1;
The average effective concentrations were the average of concentrations at 0 and 48 hours;
|Dev| = [(Cts (average) – Cnom) x 100] / Cnom
PLQ = practical limit of quantification (53.223 µg.L-1) - Details on test conditions:
- Test Conduction
In the range-finding test the exposure conditions were:
a) Total number of organisms per each tested concentration or control: 10;
b) Number of replicates per each tested concentration and control: 2;
c) Number of organisms per test container: 5;
d) Volume: 15 mL of test solution per test container, 3 mL to each Daphnia;
e) The organisms were distributed randomly in the test container. Firstly, three organisms were placed in each test container and, finally, two organisms were added to the container;
f) Nominal used concentrations: control; 0.01; 0.1; 1; 10 and 100 mg.L-1;
g) Temperature of the test solutions: 20.2 to 20.6°C;
h) Photoperiod: 16 hours of light and 8 hours of dark;
i) Parameters evaluated: immobility after 24 and 48 hours; and still animals were considered unable to swim for a period of 15 seconds of observation after mild agitation of the flasks.
In the definitive test the exposure conditions were:
a) Total number of organisms per each tested concentration or control: 20;
b) Number of replicates per each tested concentration and control: 4;
c) Number of organisms per test container: 5;
d) Volume: 15 mL of test solution per test container, 3 mL to each Daphnia;
e) The organisms were distributed randomly in the test container. Firstly, three organisms were placed in each test container and, finally, two organisms were added to the container;
f) Nominal used concentrations: control; 0.125; 0.25; 0.5; 1 and 2 mg.L-1;
g) Temperature of the test solutions: 20.3 to 20.6°C;
h) Photoperiod: 16 hours light and 8 hours dark;
i) Parameters evaluated: immobility after 24 and 48 hours; and still animals were considered unable to swim for a period of 15 seconds of observation after mild agitation of the flasks;
j) Analytical determination: content dissolved oxygen, pH of the test solutions and the test item concentration were determined at the beginning of the test and after 48 hours. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 0.71 mg/L
- Details on results:
- The value of 24-hours LC50 of Isooctyl palmitate was not determined because the highest tested concentration of 2 mg.L-1 caused only 45% immobility to organisms. The 48-hours EC50 value of 0.71 mg.L-1 (95% confidence limits = 0.58 to 0.85 mg.L-1).
In the definitive test the highest tested concentration that did not cause acute effect (immobility) to the organisms after 48 hours was 0.25 mg.L-1. The lowest concentration that caused 100% immobility was 2 mg.L-1. - Results with reference substance (positive control):
- The 24-hours EC50 value of the reference item (potassium dichromate) was 0.93 mg.L-1 (95% confidence limits = 0.79 to 1.09 mg.L-1), showing the sensitivity of the test system (ISO 6341 / OECD 202, 2004).
Any other information on results incl. tables
Criteria for Validity of the Test
The test was valid to taking care of the following conditions:
· In the control it was not exceed 10 per cent of immobilized on organism;
· The dissolved oxygen concentration at the end of the test was ≥ 3 mg.L-inthe control and the concentrations test.
Note:For the first item more then 10 per cent of daphnia did not show an immobilized in the control or others sings of disease or stress, for example, discoloration or unusual behavior such as trapping at surface of water.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-hours EC50 value of Isooctyl palmitate was 0.71 mg.L-1 (95% confidence limits = 0.58 to 0.85 mg.L-1).
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