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Diss Factsheets
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EC number: 207-256-4 | CAS number: 456-04-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11.12.1992
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: German national standard method with GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: German standard guideline: DIN 38412 part 27 (Draft)
- GLP compliance:
- yes
Test material
- Reference substance name:
- α-chloro-4-fluoroacetophenone
- EC Number:
- 207-256-4
- EC Name:
- α-chloro-4-fluoroacetophenone
- Cas Number:
- 456-04-2
- Molecular formula:
- C8H6ClFO
- IUPAC Name:
- 2-chloro-1-(4-fluorophenyl)ethan-1-one
- Details on test material:
- - Name of test material (as cited in study report): p-Fluoro-w-chloroacetophenone
- Analytical purity: > 99%
- Physical state: solid
- Lot/batch No.: 23473/86/2
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test organisms
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Laboratory culture: The test strain of Pseudomonas putida DSM 50026 used is obtained at regular intervals from DSM (German Collection of Microorganism) and is kept on agar slants for further culturing in the Ecotoxicology Laboratory of BASF AG Ludwigshafen .
- Method of cultivation:
- pre-culture
-vessel: Erlenmeyer flask
-volume: 500 mL
-inoculating density: streak with inoculating loop
-incubation time: 16 ± 2 h with shaking
-adjustment of the bacteria suspension: Centrifuge at 6000 rpm for 10 min, decant supernatant, resuspend bacteria in buffer solution and centrifuge again as stated above. Repeat washing and centrifuging. Resuspend bacteria with as little buffer solution as possible.
- Pretreatment: The concentration of the glucose stock solution is 49.54 g/100 mL water ; dissolution time 2 hours. 2 mL of this solution are added to each test and control mix.
- Initial biomass concentration: 240 - 400 TE/F (Turbidity unit/Formazin)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
Test conditions
- Test temperature:
- 21 ± 1 °C
- Details on test conditions:
- TEST SYSTEM
-vessel: Erlenmeyer flask
-volume: 500 mL
-inoculating density: streak with inoculating loop
-incubation time: 16 ± 2 h with shaking
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
OTHER TEST CONDITIONS
- Light intensity: daylight
EFFECT PARAMETERS MEASURED:
measurement of the oxygen content in the medium: 2 - 5 minutes (continuously)
cell counting: determination of the optical density of the pre-culture as validity criterion
pH-measurement: at the start of the test in all concentrations - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 30 min
- Dose descriptor:
- EC10
- Effect conc.:
- 400 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- 970 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- other: EC90
- Effect conc.:
- 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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