2-chloro-1,1-dimethoxyethane

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: OECD Guideline Study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Ethane, 2-Chloro-1,1-Dimethoxy-

IUPAC Name:

Ethane, 2-Chloro-1,1-Dimethoxy-

Constituent 2

Reference substance name:

97-97-02

IUPAC Name:

97-97-02

Constituent 3

Reference substance name:

2-chloro-1,1-dimethoxyethane

EC Number:

202-624-0

EC Name:

2-chloro-1,1-dimethoxyethane

Cas Number:

97-97-2

Molecular formula:

C4H9ClO2

IUPAC Name:

2-chloro-1,1-dimethoxyethane

Test material form:

other: liquid

Details on test material:

- Name of test material (as cited in study report): Chloroacetaldehyde dimethylacetal
- Physical state: colourless liquid
- Solubility in water: 52 g/L at 20°C
- pH: 3.4 at 10g/L (20°C)

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra danio
- Source: West Aquarium, D-Bad Lauterberg
- Length at study initiation: 2-3cm
- Weight at study initiation: 0.35g in average
- Feeding during test: no

ACCLIMATION
- Acclimation period: 7d in test water befor study initiation
- Acclimation conditions (same as test or not): yes
- Type and amount of food: flakes (Tetra Min Hauptfutter)
- Feeding frequency: 3 times/week, last feeding 24h before study initiation
- Health during acclimation (any mortality observed):

QUARANTINE
- Duration: 12d in quarantine
- Health/mortality: no external visible illnesses/0.25%

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96

Remarks on exposure duration:

h

Test conditions

Hardness:

2.83 - 3.14 mmol/L

Test temperature:

21 +/- 1°C

pH:

7.39-7.49

Nominal and measured concentrations:

nominal: 0, 25, 50, 100, 200, 400 mg/L
measured: 0, 26.31/24.76, 47.78/48.84, 99.46/92.11, 205.48/204.58, 405.81/396.37 mg/L

Details on test conditions:

TEST SYSTEM
- Material, size, headspace, fill volume: 10L
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): every 24h
- No. of organisms per vessel: 10

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tab water
Dilution water:
CaCl2: 2 mmol/L, MgSO4: 0.5 mmol/L, NaHCO3: 0.75 mmol/L, KCl: 0.075 mmol/L
The sum of the Calcium- and Magnesium ions in the dilution water is 2.5 mmol/L. The relation of Ca : Mg ions 4 : 1 and Na : K ions 10 : 1. The acid capacity of the solution is 0.8 mmol/L. The water will be aerated until oxygen saturation; afterwards the stock solutions will be mixed with 500mL dilution water.

Stock solutions:
(1): 0.5 g/L Chloroacetaldehyde dimethylacetal, (2): 1 g/L Chloroacetaldehyde dimethylacetal, (3): 2 g/L Chloroacetaldehyde dimethylacetal, (4) 4 g/L Chloroacetaldehyde dimethylacetal, (5): 8 g/L Chloroacetaldehyde dimethylacetal.
stock solution : dilution water is 1 : 20

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 light/8 dark
- Light intensity: 2000 lux

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Details on results:

- Behavioural abnormalities: balance disorders in 1 fish (24h) and 2 fish (48h)
- Observations on body length and weight: normal: 2,8 cm in average and 0.35g in average
- Mortality of control: no
- Other adverse effects control: no

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Chloroacetaldehyde dimethylacetal was tested according to OECD Guideline 203 tested in zebra danio (Brachydanio rerio (new name: Danio rerio)). 2 out of 6 died, so the mortality was 1/3 at a concentration of 100 mg/l.

Executive summary:

Chloroacetaldehyde dimethylacetal was tested according to OECD Guideline 203 tested in zebra danio (Brachydanio rerio (new name: Danio rerio)). The LD50 is > 100 mg/l.