2-dibutylaminoethanol

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well-documented report which meets basic scientific principles.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1967

Report date:

1967

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Standardised study to determine the acute toxicity of the test item in mice after single i.p. application.

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-dibutylaminoethanol

EC Number:

203-057-1

EC Name:

2-dibutylaminoethanol

Cas Number:

102-81-8

Molecular formula:

C10H23NO

IUPAC Name:

2-(dibutylamino)ethan-1-ol

Details on test material:

- Name of test material (as cited in study report): Dibutylaethanolamin
- Analytical purity: >99%

Test animals

Species:

mouse

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 29 g (mean males), 24 g (mean females)
No further data.

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

other: aqueous emulsion with traganth

Details on exposure:

MAXIMUM DOSE VOLUME APPLIED: The doses were administered as aqueous emulsions with traganth of 1% (25, 50, 100, 125, 160 mL/kg), 2% (200 mL/kg) and 20% (1600 mL/kg).

Doses:

25, 50, 100, 125, 160, 200, 1600 mL/kg bw (22, 43, 86, 108, 138, 172, 1376 mg/kg bw - conversion in mg/kg is based on the density: d= 0.8601 g/cm³).

No. of animals per sex per dose:

5

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 7 days
- Frequency of observations: daily
- Frequency of weighing: before the start of the study
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 138 mg/kg bw

Based on:

test mat.

Remarks on result:

other: Corresponds to 125/160 mL/kg bw; the mg/kg value was calculated by means of the density: d= 0.8601 g/cm³. 138 mg/kg bw: 4 female and 2 male animals died within 1 hour post application.

Mortality:

1376, 172 mg/kg bw: all animals died within 1 hour post application
138 mg/kg bw: 4 female and 2 male animals died within 1 hour post application
108 mg/kg bw: 1 male and 1 female animal died within 1 hour post application
22, 43, 86 mg/kg bw: no death

Clinical signs:

1376, 172 mg/kg bw: Immediately after application convulsion, sounds of pain, dyspnoea was observed until death.
138 - 22 mg/kg bw: Immediately the animals slowed-down and closed the eyes. Intermittent respiration, tremor, sounds of pain, secretion out of the oral cavity was observed. On the next day, accelerated respiration, ruffled fur and glued eyes were observed. 2 - 6 days after injection, the animals recovered.

Body weight:

No data on body weight gain.

Gross pathology:

Animals that died: conglutination and adhesion (particularly in the area of the liver), fibrosis of the liver capsules, adhesive peri-hepatitis.
No abnormalities were detected in the animals that were sacrificed at the end of the study.