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EC number: 259-393-4 | CAS number: 54914-37-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: samples taken from additional beakers without daphnia, kept under the same experimental conditions
- Sample storage conditions before analysis: samples were taken, immediately transferred to the analytical laboratory and analysed on the same day. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Test substance concentrations were individually prepared in M4 medium, stirred for 24 h, and afterwards the preparations were filtered .
- Controls: control preparations without test substance but treated the same way. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna, water flea
- Source: Landesumweltamt NRW, Düsseldorf, Germany
- Age at study initiation (mean and range, SD): < 24 h
- Method of breeding: The daphnids were held in beakers with a nominal capacity of 2000 mL. Each beaker was filled with approximately 1400 mL M4 medium (Elendt-Schneider). Between 15 and 20 adult organisms were held in one beaker in order to get a high reproduction rate and viable offspring. The breeding beakers were kept at an additional artificial light-dark cycle of 16 to 8 hours. Breeding beakers were placed in a temperature controlled laboratory at 20 +/- 2°C. The breeding medium was renewed three times a week.
- Feeding during test: no feeding during experimental period
ACCLIMATION
- Acclimation period: culture of daphnids permanently kept at the test facility
- Acclimation conditions (same as test or not): no acclimation necessary
- Type and amount of food: singel cell green algae (Desmodesmus subspicatus)
- Feeding frequency: daily - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 2.502 mmol/L (total hardness)
- Test temperature:
- 20.7 - 22.0°C
- pH:
- 7.95 - 8.28
- Dissolved oxygen:
- 5.5 - 6.2 mg/L
- Nominal and measured concentrations:
- nominal: 3.75 mg/L, 7.5 mg/L, 15 mg/L, 30 mg/L, 60 mg/L
measured (DOC at 48 h): 20.5 mg/L (nominal 30 mg/L), 31.4 mg/L (nominal 60 mg/L) - Details on test conditions:
- TEST SYSTEM
- Type: open
- Material, size, headspace, fill volume: glas test beakers (50 mL), filled with 30 mL
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 5
- Biomass loading rate: 1 daphnid per 6 mL test solution
TEST MEDIUM / WATER PARAMETERS
- Total organic carbon: 0 mg/L
- Alkalinity: 0.936 mmol/L
- Conductivity: 588 µS/cm
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Photoperiod: 16 h / 8 h light / dark cycle
- Light intensity: 700 +/- 50 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobility
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Results of range finding study used to determine the conditions for the definitive study - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 22.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 17.0-30.2
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 14.7 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- dissolved
- Remarks:
- (DOC)
- Basis for effect:
- mobility
- Remarks on result:
- other: 4.9-20.2
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 7.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: no immobility observed in the control group
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- - Results with reference substance valid? yes, test was conducted on 22 February 2011 (24 h)
- Measured parameter: immobility
- EC50/LC50: EC50 after 24 h = 1.0 mg/L; in the required range of 0.6 - 2.1 mg/L - Reported statistics and error estimates:
- EC values calculated with ToxRat Professional (ToxRat Solutions GmbH, Alsdorf, Germany)
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 (48 h), based on nominal concentrations was determined as 22.2 mg/L indicating that the test substance may be harmful to aquatic invertebrates. Based on DOC, the EC50 (48 h) was 14.7 mg/L.
- Executive summary:
The test substance was tested in the Acute Daphnia Toxicity Test according to the OECD Guideline for the Testing of Chemicals No. 202 (April 2004) using Daphnia magna (Straus) as test organism. The experiment was carried out as a static test, i.e. the test substance preparations were not exchanged for the whole exposure period of 48 hours. The test was performed with test substance preparations of nominal 3.75 mg/L, 7.5 mg/L, 15 mg/L, 30 mg/L, 60 mg test substance/L M4 medium. The EC50value after 48 hours of exposure was 22.2 mg/L and the NOEC was 7.5 mg/L, based on nominal concentrations. Based on measured DOC values, the EC50value was 14.7 mg/L.
Reference
Analytical data: see attachment
Effect values:
EC value | Effective concentration after 48 hours [mg/L] |
| nominal |
EC50 | 22.2 |
95 % Confidence interval | 17.0 – 30.2 |
NOEC | 7.5 |
Vessel No. | Concentration of test substance (mg/L) | Immobilised daphnids per test vessel | |||||||||||
| 24 h | 48 h | |||||||||||
1 - 5 | 0 (Control) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
6 - 10 | 3.75 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
11 - 15 | 7.5 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | ||
16 - 20 | 15 | 3 | 2 | 2 | 2 | 2 | 3 | 2 | 2 | 2 | 2 | ||
21 - 25 | 30 | 0 | 2 | 3 | 2 | 4 | 2 | 2 | 3 | 3 | 5 | ||
26 - 30 | 60 | 3 | 3 | 2 | 3 | 2 | 4 | 4 | 4 | 4 | 4 |
Description of key information
The test substance was tested in the Acute Daphnia Toxicity Test according to the OECD Guideline for the Testing of Chemicals No. 202 (April 2004) using Daphnia magna (Straus) as test organism. The experiment was carried out as a static test, i.e. the test substance preparations were not exchanged for the whole exposure period of 48 hours. The test was performed with test substance preparations of nominal 3.75 mg/L, 7.5 mg/L, 15 mg/L, 30 mg/L, 60 mg test substance/L M4 medium. The EC50value after 48 hours of exposure was 22.2 mg/L and the NOEC was 7.5 mg/L, based on nominal concentrations. Based on measured DOC values, the EC50 value was 14.7 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 14.7 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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