Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-770-8 | CAS number: 110-46-3
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- ''amyl nitrite'', mixed isomers
- EC Number:
- 203-770-8
- EC Name:
- ''amyl nitrite'', mixed isomers
- Cas Number:
- 110-46-3
- Molecular formula:
- C5H11NO2
- IUPAC Name:
- pentyl nitrite
Constituent 1
Test conditions
- Test temperature:
- 23 ± 2°C
- Nominal and measured concentrations:
- Target concentrations (mg.L-1)
Control
Solvent control
0.3
0.95
3.05
9.77
31.25
100 - Details on test conditions:
- Light: continuous illumination between 4440 and 8880 lux
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.14 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.53 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- biomass
- Remarks:
- yield
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.15 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.11 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- biomass
- Remarks:
- yield
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The objective of this study was to assess the effects of test item Isoamyl Nitrite on the growth of Pseudokirchneriella subcapitata over a period of 72 hours according to OECD 201 Guideline. Pseudokirchneriella subcapitata is a freshwater green algae that is recommended by the OECD Guideline 201.
Since the substance is volatile and is not soluble in under conditions of the test, the test was carried out in closed bottles and the substance was added by injection through the septum.
Test item quantification was performed according to a validated analytical method (final report RRCo-000234_01) on a GC-MS/MS from Agilent (GC 7890b and MS triple quadrupole 7000b or c) as mass spectrometry detection was found suitable to measure Isoamyl Nitrite concentrations at 0, 24, 48 and 72 hours.
The effects of Isoamyl Nitrite at the definitive nominal concentrations of 0; 0.3; 0.95; 3.05; 9.77; 31.25 and 100 mg.L-1 to Pseudokirchneriella subcapitata were investigated under laboratory conditions.
Due to the instability of test item, the calculations were performed on the nominal concentrations.
Results are the following:
Isoamyl Nitrite
ECgrowth rate 10-72h (95% confidence limits): 0.15 mg.L-1 (95% confidence limits: 0.05 – 0.30 mg.L-1)
ECgrowth rate 50-72h (95% confidence limits): 2.14 mg.L-1 (95% confidence limits: 1.59 – 3.33 mg.L-1)
ECyield 10-72h (95% confidence limits): 0.11 mg.L-1 (95% confidence limits: 0.02 – 0.22 mg.L-1)
ECyield 50-72h (95% confidence limits): 0.53 mg.L-1 (95% confidence limits: 0.31 – 0.75 mg.L-1)
EC = effect concentration
The study is valid since the growth multiplication factor in the control within the 72-hour test period was higher than 16 (actual value: 17), the coefficient of variation of the average specific growth rates in the control was less than 7% (actual value: 6.89%)
The mean coefficient of variation for section-by-section specific growth rates (days 0-1, 1-2 and 2-3) in the control was not reached (actual value: 67.08% after 72h, the criteria is fixed at < 35%). Indeed, the bottles being sacrificial, the daily monitoring was not on the same bottle. It is therefore not significant to follow the growth of a replicate in time because it is not the same bottle.
This is no impact on the study, the other criteria were reached, so the study is valid.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
