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EC number: 204-472-0 | CAS number: 121-46-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- August 2017 - January 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 439 (In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 8,9,10-trinorborna-2,5-diene
- EC Number:
- 204-472-0
- EC Name:
- 8,9,10-trinorborna-2,5-diene
- Cas Number:
- 121-46-0
- Molecular formula:
- C7H8
- IUPAC Name:
- bicyclo[2.2.1]hepta-2,5-diene
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material: AIR LIQUIDE ELECTRONICS MATERIALS Batch N° 10006042
- Purity: 99.8%
- Expiry date: 16 August 2018
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature
- Stability and homogeneity of the test material under test conditions and during storage: stable
In vitro test system
- Test system:
- human skin model
- Source species:
- human
- Cell type:
- non-transformed keratinocytes
- Cell source:
- foreskin from a single donor
- Amount/concentration applied:
- 16 µL
- Duration of treatment / exposure:
- 42 mins
- Duration of post-treatment incubation (if applicable):
- 42h
- Number of replicates:
- 3
Results and discussion
In vitro
Results
- Irritation / corrosion parameter:
- % tissue viability
- Run / experiment:
- Mean
- Value:
- 6.8
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
Skin | OD | Mean OD / disc (#) | Mean OD/ product | Viability | Mean viability | SD viability | Conclusion | |
Negative control | 1 | 0.772 | 0.810 | 0.653 | 124.0 | 100.0 | 20.8 | |
0.824 | ||||||||
0.834 | ||||||||
2 | 0.555 | 0.579 | 88.6 | |||||
0.595 | ||||||||
0.587 | ||||||||
3 | 0.542 | 0.571 | 87.4 | |||||
0.591 | ||||||||
0.579 | ||||||||
Positive control | 4 | 0.014 | 0.016 | 0.012 | 2.4 | 1.8 | 0.6 | Irritant |
0.018 | ||||||||
0.015 | ||||||||
5 | 0.011 | 0.011 | 1.7 | |||||
0.010 | ||||||||
0.010 | ||||||||
6 | 0.009 | 0.009 | 1.4 | |||||
0.008 | ||||||||
0.008 | ||||||||
Test item PH-17/0495 | 10 | 0.034 | 0.034 | 0.045 | 5.2 | 6.8 | 5.2 | Irritant or Corrosive |
0.034 | ||||||||
0.032 | ||||||||
11 | 0.018 | 0.017 | 2.6 | |||||
0.016 | ||||||||
0.015 | ||||||||
12 | 0.082 | 0.083 | 12.7 | |||||
0.080 | ||||||||
0.087 |
Applicant's summary and conclusion
- Interpretation of results:
- Category 1 (corrosive) based on GHS criteria
- Conclusions:
- In accordance with the Regulation EC No. 1272/2008 and in absence of information on skin corrosion, the test item BCHD-Bicyclo 2,2,1-Hepta 2,5 diene has to be classified in Category 2 "Irritating to skin" or in Category 1 "Corrosive". The hazard statement "H315: Causes skin irritation" with the signal word "Warning" or "H314: Causes severe skin burns and eye damage" with the signal word "Danger" are required.
In absence of further information, the precautionary classification as Category 1 "Corrosive" was chosen. - Executive summary:
The aim was to evaluate the possible irritating effects of the test item BCHD-Bicyclo 2,2,1-Hepta 2,5 diene after topical application on in vitro human reconstructed epidermis (SkinEthic RHE@ model).
The test item BCHD-Bicyclo 2,2,1-Hepta 2,5 diene was applied as supplied, at the dose of 16 µL, to 3 living Reconstructed Human epidermis (SkinEthic RHE® model) during 42 minutes. The application was followed by a rinse with 25 mL of DPBS and a 42 hours and 40 minutes postincubation period at 37°C, 5% CO2. Cell viability was then measured by enzymatic conversion of the vital dye MTT into a blue formazan salt that was quantitatively measured after extraction from tissues. The experimental protocol was established in accordance with O.E.C.D. Test Guideline No. 439 adopted 28 July 2015 and the Test method B.46 of Council regulation No. 761/2009 dated 23 July 2009 (EU Journal L220) - ATP Council regulation No. 440/2008 of 30 May 2008 (E.U. Journal L142).
The mean percent viability of the treated tissues was 6.8%, versus 1.8% in the positive control (5% Sodium Dodecyl Sulfate).
In accordance with the Regulation EC No. 1272/2008 and in absence of information on skin corrosion, the test item BCHD-Bicyclo 2,2,1-Hepta 2,5 diene has to be classified in Category 2 "Irritating to skin" or in Category 1 "Corrosive". The hazard statement "H315: Causes skin irritation" with the signal word "Warning" or "H314: Causes severe skin burns and eye damage" with the signal word "Danger" are required
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