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Environmental fate & pathways

Adsorption / desorption

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Reference
Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
November - December 2014
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
Deviations:
no
Qualifier:
according to
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Deviations:
no
GLP compliance:
yes (incl. certificate)
Type of method:
HPLC estimation method
Media:
suspended matter
Specific details on test material used for the study:
TEST MATERIAL
- Substance identification/name in the report: LZ 596
- Molecular formula: C21H30N2
- Batch no.: 1229
- Analysis date: August 12, 2014
- Date of production: August 11, 2014

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage conditions: Controlled room temperature (15-25ºC, <70 RH%), protected from light and humidity.
- Stability under test conditions: stable for min. 3 years from manufacturing date
- Expiry date: August 10, 2017
- Safety precautions: Routine safety precautions (lab coat, gloves, safety glasses, face mask) for unknown materials were applied to assure personnel health and safety.
Radiolabelling:
no
Test temperature:
room temperature
Details on study design: HPLC method:
APPARATUS:
- Stationary Phase: LiChrospher 100 CN, 5 μm, 250 × 4 mm, No: 933278
- HPLC system: Merck-Hitachi LaChrom Elite
L-2000 Organizer, No.: 23E94-011
L-2130 HPLC pump, No.: 23E60-025
L-2200 Autosampler, No.: 23E91-010
L-2400 UV Detector, No.: 23E88-005
L-2350 Column Oven, No.: 2383-009
- Balances: BL 120S, Sartorius, No.: 15307011 PG 203 S,
Mettler Toledo, No.: 1122082152 AB54-S,
Mettler Toledo, No.: 1122092721
- pH meter: HACH, HQ40d, No.: 090400030482
- Ultrasonic bath: Branson 3210E-MT, No.: A700343D
- Water purification system: MILLIPORE, DIRECT Q5, No.: F0DA13956K

CONDITIONS:
- Mobile Phase: Methanol / 0.01M Citrate-buffer pH 6.0 (55/45% v/v)
- Flow: 1.0 mL/min
- Injection volume: 5 μL
- Column temperature: 25°C
- Detection wavelength: 254 nm, 210 nm in case of Sodium nitrate
Key result
Type:
Koc
Value:
6 990 dimensionless
Temp.:
20 °C
Matrix:
HPLC-system
Remarks on result:
other: mean value; pH not measured
Key result
Type:
log Koc
Value:
3.8 dimensionless
Temp.:
20 °C
Matrix:
HPLC-system
Remarks on result:
other: mean value; pH not measured
Details on results (HPLC method):
Accuracy and repeatability of the calibration points

Chemical name Reference log KOC Retention time (min) log k log KOC (back calculated) Accuracy log unit Repeatability log unit
Acetanilide 1.25 3.35 -0.232 1.00 0.25 0.000
Methyl benzoate 1.80 3.69 -0.097 1.63 0.17 0.012
Naphthalene 2.75 5.20 0.187 2.95 0.20 0.013
Fenthion 3.31 6.70 0.356 3.74 0.43 0.009
Phenanthrene 4.09 7.85 0.452 4.19 0.10 0.007
4,4’-DDT 5.63 12.14 0.693 5.31 0.32 0.010

Validity criteria fulfilled:
yes
Conclusions:
The estimated log Koc value of the test item was determined to be 3.8. The estimated Koc is 6990.
Executive summary:

The test item was investigated for its adsorption/desorption behaviour in a screening test, in accordance with EC-method C.19 (HPLC-method). The estimated log Koc value of the test item was determined to be 3.8. The estimated Koc is 6990.

Description of key information

The test item was investigated for its adsorption/desorption behaviour in a screening test, in accordance with EC-method C.19 (HPLC-method). The estimated log Koc value of the test item was determined to be 3.8. The estimated Koc is 6990.

Key value for chemical safety assessment

Koc at 20 °C:
6 990

Additional information

Source: GLP-report

[LogKoc: 3.8]