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EC number: 500-345-1 | CAS number: 157627-95-7 1 - 2.5 moles ethoxylated
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 04 - 08 Nov 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- (2010)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: test substance was warmed up in a water bath at 39.6 °C. Container was then shaken. Direct addition by initial weight into the test flasks followed. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Source: municipal wastewater treatment plant of Mannheim, Germany
- Preparation of inoculum for exposure: after arrival of the activated sludge suspension in the test facility the suspension was sieved with a fine woven mesh (mesh size about 1 mm). Suspension was pre-aerated over night at room temperature. At the next day sludge suspension was washed once with drinking water and adjusted to 3 g/L dw
- Initial biomass concentration: 1.5 g/L dw - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 21.2 - 21.5 °C
- pH:
- 7.4 - 7.5 (initial pH)
7.9 - 8.0 (pH at the end) - Dissolved oxygen:
- 7.2 - 8.0 mg/L (test start)
- Nominal and measured concentrations:
- 0 (control), 182, 364, 729, 1458, 2915 mg/L (nominal test concentrations)
0 (control), 62.5, 125, 250, 500 and 1000 mg/L (a.i.) - Details on test conditions:
- TEST SYSTEM
- Test vessel: 1 L glas beakers
- Fill volume: 500 mL (16 mL 100-fold concentrated synthetic medium with either test substance or reference substance + 234 mL deionized water + 250 mL inoculum suspension)
- Aeration: after preparation of the vessels incubation started by aeration of the vessels with pressured air
- Sludge concentration: 1.5 g/L dw
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per positive control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Intervals of water quality measurement: temperature was measured 8 times during the incubation in a separate test vessel with deionized water
EFFECT PARAMETERS MEASURED: oxygen uptake was measured for a period of about 8 to 10 min. Due to foaming of the test substance solution in the test vessel during aeration, 25 μL of triisobutyl sulfate was added in all test vessels as antifoam agent - Reference substance (positive control):
- yes
- Remarks:
- 3,5 dichlorophenol (1, 10, 100 mg/L)
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 2 915 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 140 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 410 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- - Results with reference substance valid: yes
- Relevant effect levels: EC50 = 3.5 mg/L - Reported statistics and error estimates:
- O2 consumption rates were used for the determination of the ECx by the probit method with the software TOXRAT Professional 2.10.
- Validity criteria fulfilled:
- yes
- Remarks:
- Please refer to tabe 1 at "Any other information on results incl. tables"
Reference
EC80 > 1000 mg/L based on active ingredient (2915 mg/L based on nominal effect concentration)
Due to technical problems the measured values of the oxygen consumption after 3 hours of incubation of blank control 2 were lost.
Table 1: Validity criteria for OECD 209
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The blank controls (without the test substance or reference substance) oxygen uptake rate should not be less than 20 mg oxygen per one gram of activated sludge (dry weight of suspended solids) in an hour. |
20 mg/g*h |
yes |
The coefficient of variation of oxygen uptake rate in control replicates should not be more than 30% at the end of definitive test. |
14.1% |
yes |
The EC50 of 3,5-dichlorophenol should be in the range of 2-25 mg/L |
3.5 mg/L |
yes |
Description of key information
One study investigating the toxicity ofAlcohols, C16-18 and C18-unsatd., ethoxylated, sulfates, sodium saltsto activated sludge microorganisms is available. The study was performed according to OECD guideline 209 (GLP) using activated sludge from a municipal wastewater treatment plant. The respiration inhibition of the microorganisms at five different item concentrations (62.5, 125, 250, 500 and 1000 mg/L a.i.) was measered for three hours. An EC50 (3h) > 1000 mg/L a.i. (nominal) was determined.
Key value for chemical safety assessment
Additional information
The toxicity of the alkyl ether sulfates (AES) substances to microorganisms was assessed by read-across based on the grouping of substances (category approach). All data on toxicity to microorganisms available for the category were used to assess the toxicity of category substances to microorganisms. The available data indicate no adverse effects of alkyl ether sulfates on microbial activity. All substances of the alkyl ether sulfates category are considered readily biodegradable. In the available screening studies including a toxicity control, no inhibition of the degradation process by the test substance was observed. The available studies investigating the respiration inhibition of activated sludge organisms and Pseudomonas putida determined no detrimental effects of the tested alkyl ether sulfates.
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