Dimethyl sulphone

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from handbook.

Data source

Reference

Reference 1

Reference Type:

review article or handbook

Title:

Acute dermal toxicity study of test chemical in rodents.

Author:

Richard J. Lewis

Year:

2012

Bibliographic source:

Sax's Dangerous Properties of Industrial Materials

Reference 2

Reference Type:

review article or handbook

Title:

Acute Toxicity - TEST SUBSTANCE

Author:

John Wiley & Sons

Year:

2005

Bibliographic source:

Patty's Industrial Hygiene and Toxicology

Reference 3

Reference Type:

other: authoritative database

Title:

Acute Dermal toxicity - Test chemical

Author:

U.S. National Library of Medicine

Year:

2018

Bibliographic source:

ChemIDplus

Reference 4

Reference Type:

other: Authoritative database

Title:

Toxicity of test chemical

Author:

IFA GESTIS

Year:

2018

Bibliographic source:

GESTIS Substance Database

Reference 5

Reference Type:

secondary source

Title:

Acute dermal toxicity study of test chemical in rabbits

Author:

NTRL

Year:

1991

Bibliographic source:

NTRL report

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: As mentioned below

Principles of method if other than guideline:

The toxicity study was examined to evaluate the acute toxic effects of administration of the test chemical in male New Zealand White rabbits by the dermal route.

GLP compliance:

not specified

Test type:

other: not specified

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Dimethyl sulphone

EC Number:

200-665-9

EC Name:

Dimethyl sulphone

Cas Number:

67-71-0

Molecular formula:

C2H6O2S

IUPAC Name:

dimethyl sulphone

Details on test material:

- IUPAC Name: Dimethyl sulphone
- InChI: 1S/C2H6O2S/c1-5(2,3)4/h1-2H3
- Smiles: S(C)(C)(=O)=O
- Molecular formula :C2H6O2S
- Molecular weight :94.1334 g/mole
- Substance type:Organic
- Physical state:Solid, crystalline white odorless

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Skippack Farms
- Weight at study initiation: mean wt: 2.23

Administration / exposure

Type of coverage:

occlusive

Vehicle:

physiological saline

Details on dermal exposure:

TEST SITE
- Area of exposure: The closely clipped skin.
- Type of wrap if used: The test chemical was held under an impervious cuff.

REMOVAL OF TEST SUBSTANCE
- Washing (if done): The cuffs were removed and the application sites were gently wiped to remove the test substance.
- Time after start of exposure:24-hr

TEST MATERIAL
- For solids, paste formed: yes, the test substance, prepared as a paste in saline (1:1)

Duration of exposure:

24 hours

Doses:

5000 mg/kg bw

No. of animals per sex per dose:

Total = 2

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days
- Other examinations performed: Animals were observed for mortality and clinical signs.
- Necropsy of survivors performed: yes

Statistics:

not specified

Results and discussion

Preliminary study:

not specified

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Based on:

test mat.

Mortality:

No mortality was observed at the dose concentration of 5000 mg/kg bw.

Clinical signs:

other: Well defined erythema and very slight edema was observed.

Gross pathology:

No gross changes were observed in either rabbit.

Other findings:

not specified

Applicant's summary and conclusion

Interpretation of results:

other: Not classified

Conclusions:

The acute dermal LD50 value was considered to be >5000 mg/kg bw, when 2 male New Zealand White rabbits were treated with the given test chemical by dermal application occlusively.

Executive summary:

The acute dermal toxicity study was conducted by using the given test chemical in 2 male New Zealand White rabbits at the dose concentration of 5000 mg/kg bw.

The test substance, prepared as a paste in saline (1:1), was held under an impervious cuff in continuous 24 hr contact with the closely clipped skin. After the 24-hr exposure, the cuffs were removed and the application sites were gently wiped to remove the test substance. Animals were observed for mortality and clinical signs. Necropsy of survivors performed.

No mortality was observed at the dose concentration of 5000 mg/kg bw. Well defined erythema and very slight edema was observed. No gross changes were observed in either rabbit.

Hence, the LD50 value was considered to be >5000 mg/kg bw, when 2 male New Zealand White rabbits were treated with the given test chemical by dermal application occlusively.