Registration Dossier
Registration Dossier
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EC number: 215-609-9 | CAS number: 1333-86-4
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 977
- Report date:
- 1977
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Deviations:
- no
- GLP compliance:
- no
Test material
- Reference substance name:
- Carbon black
- EC Number:
- 215-609-9
- EC Name:
- Carbon black
- Cas Number:
- 1333-86-4
- Molecular formula:
- C
- IUPAC Name:
- carbon
- Test material form:
- solid: nanoform, no surface treatment
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS: albino rabbits, New Zealand strain, average weight 2.3-2.8 kg. 3 male, and 3 female animals.
HOUSING: stainless steel cages, 1 animal/cage, standard diet (Altromin), water ad lib., temperature 21 +/- 2 deg C, relative humidity 60 +/- 3%, 12 hours artificial light/12 hours dark.
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: other eye
- Amount / concentration applied:
- undiluted
Amount applied: 100 mg - Duration of treatment / exposure:
- EXPOSURE TO THE TEST SUBSTANCE: 100 mg of the test substance was instilled into the left eye. The other eye was treated with physiological saline solution and served as control. The eyes were not rinsed.
- Observation period (in vivo):
- OBSERVATIONS: eyes were evaluated for erythema, edema, and effects on cornea and iris according to the Draize score at 24, 48, 72 and 96 hours after instillation.
- Number of animals or in vitro replicates:
- 6
- Details on study design:
- Comment: not rinsed
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Remarks:
- 6 animals
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Irritation parameter:
- iris score
- Basis:
- mean
- Remarks:
- 6 animals
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Remarks:
- 6 animals
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 3
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Remarks:
- 6 animals
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Irritant / corrosive response data:
- No irritant effects were found in any of the animals at any observation (scores for cornea, iris and conjunctivae: 0.0, 0.0, 0.0).
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- not an eye irritant
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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