1,1,1-trifluoroethane

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP laboratory study from original report

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

1,1,1-trifluoroethane

EC Number:

206-996-5

EC Name:

1,1,1-trifluoroethane

Cas Number:

420-46-2

Molecular formula:

C2H3F3

IUPAC Name:

1,1,1-trifluoroethane

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

Concentrations of test substance in solution were o (control), 1.3, 2.5, 6.4, 9.5, 22, and 40 mg/l.

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Groups of 10 unsexed trout approx. 3.3 cm in length and 0.526 gms.

Study design

Test type:

flow-through

Water media type:

freshwater

Total exposure duration:

96 h

Post exposure observation period:

none

Test conditions

Hardness:

data not available, water obtained from a 360 well

Test temperature:

12.0 to 12.3 deg C

pH:

data not available

Dissolved oxygen:

assumed to be at or near saturation. Air bubbled continuously through the water

Salinity:

data not available

Nominal and measured concentrations:

Nominal Concentrations (mg/l) 0(control), 11, 22, 44, 88, 175, and 350 while the Average Measured concentrations were (mg/l) 0.3, 1.3, 2.5, 6.4, 9.5, 22, and 40, respectively. This large difference between nominal concentrations and measured concentrations is the result of testing a highly volatile material (boiling point -47.4 deg C) that is only slightly soluble in an aqueous system.

Details on test conditions:

The large differences between nominal and measured concentrations result from losses of test substance due to the high volatility (boiling point -47.4 deg C) and relatively low solubility in water.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC10

Effect conc.:

ca. 40 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks:

1 fish died at 72 hrs

Details on results:

The large difference between nominal concentrations and measured concentrations is the result of testing a highly volatile material (boiling point -47.4 deg C) that is only slightly soluble in an aqueous system.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 96-hr LC50 in rainbow trout was determined to be > 40 mg/l (analytically measured by GC) The nominal concentration for this level was 350 mg/l. This large difference between nominal concentrations and measured concentrations is the result of testing a highly volatile material (boiling point -47.4 deg C) that is only slightly soluble in an aqueous system and rapidly is lost from the test system. It is therefore concluded that the calculated value of 109 mg/L is the more accurate value.

Executive summary:

The 96-hr LC50 in rainbow trout was determined to be > 40 mg/l (analytically measured by GC) The nominal concentration for this level was 350 mg/l. This large difference between nominal concentrations and measured concentrations is the result of testing a highly volatile material (boiling point -47.4 deg C) that is only slightly soluble in an aqueous system and rapidly is lost from the test system. It is therefore concluded that the calculated value of 109 mg/L is the more accurate value.