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Toxicological information

Basic toxicokinetics

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Administrative data

Endpoint:
basic toxicokinetics in vivo
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
publication
Title:
Metabolism of labeled 2-arbon Acids in the intact rat.
Author:
Weinhouse S and Friedmann B
Year:
1951
Bibliographic source:
Biological Chemistry 191: 707-717

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
C14 labelled Glyoxylic Acid (0.06 mmol) was administered to rats by i.p. injection together with sodium-benzoate. Doses totaling 1 mmol of sodium-benzoate with the labelled acid as the sodium salt were administered. The rate of oxidation products in urine and the appearance of C-14 activity in exhaled CO2 in urine was studied.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Radiolabelling:
yes

Test animals

Species:
rat
Strain:
not specified
Sex:
male

Administration / exposure

Route of administration:
intraperitoneal

Results and discussion

Main ADME results
Type:
excretion
Results:
16 % and 49.1% of applied radioactivity exhaled and excreted in urine, respectively.

Toxicokinetic / pharmacokinetic studies

Details on excretion:
The rate of oxidation as indicated by the appearance of C-14 activity in carbon-dioxide (CO2) was studied at 0.5 hour intervals for 5 hours. Rates of oxalic acid and hippuric acid formation were measured in urine for 24 hours. Glyoxylic Acid was excreted to 16 % in respiratory CO2, 27.1 % as oxalic acid and 22 % as hippuric acid in the urine.

Metabolite characterisation studies

Metabolites identified:
yes
Details on metabolites:
16 % in respiratory CO2, 27.1 % as oxalic acid and 22 % as hippuric acid in the urine.

Applicant's summary and conclusion