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Administrative data

Endpoint:
additional toxicological information
Type of information:
other: Literature data
Adequacy of study:
other information
Study period:
1993
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Literature data

Data source

Reference
Reference Type:
publication
Title:
The absence of glyoxylate cycle enzymes in rodent and embryonic chick liver
Author:
Holmes RP
Year:
1993
Bibliographic source:
Biochimica et Biophysica Acta, 1158, 47-51.

Materials and methods

Type of study / information:
Absence of glyoxylate cycle enzymes in rodent and embryonic chick liver.
Principles of method if other than guideline:
The aim of this study was to determine the absence of glyoxylate cycle enzymes in rodent and embryonic chick liver.
Reaction products in these assays have been measured principally by chromatographic separation of isotopes or by colorimetric procedures.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent

Results and discussion

Any other information on results incl. tables

Reversed phase HPLC revealed the absence of detectable isocitrate lyase activity in guinea pig, rat and chick embryonic liver. The formation of several other alpha-keto acids was detected and this may account for the previously reported activities.
Using HPLC to monitor malate formation malate synthase activity was not detected in these tissues.

Applicant's summary and conclusion

Conclusions:
These results indicate that when assaying enzyme activities in crude tissue homogenates specific methods for the identification of end products are required.