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Short-term toxicity to fish

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Description of key information

The short-term LC50 value for fish was based upon the 96-hour LC50 > 100 mg/L (nominal concentration;  pH-adjusted) in Leuciscus idus reported in the key study (BASF AG, 1988).  

Key value for chemical safety assessment

LC50 for freshwater fish:
100 mg/L

Additional information

The acute toxicity of PACM (CAS 1761 -71 -3) was studied in a static test according to German Industrial Standards DIN 38412, part 15 usingLeuciscus idusas test species (BASF AG, 1988; report no. BASF AG 10F0562/875273). The test concentrations were not analytically verified, but are assumed to be stable regarding the low biodegradadation potential and the low Henry's Law Constant of 9.6E-08 Pa*m³/mol at pH 7.

The 96 -h LC50 was determined to be > 100 mg/L (nominal concentration; pH-adjusted solution). The study also reported a 96 -h

LC50 of 67.8 mg/L from the non-pH-adjusted series of test concentrations (nominal; geometric mean; not pH-adjusted). The toxic effect was significantly reduced after pH-adjustment (at 100 mg/L: 100% mortality without pH adjustment, 10% mortality after pH adjustment). The 96-hour LC50> 100 mg/L for Leuciscus idus was the representative value for the short-term toxicity of 4,4'-methylenebis(cyclohexylamine) to fish.

This value represented one of the three short-term aquatic toxicity values for each of three trophic levels (fish, aquatic invertebrates and aquatic algae and plants). The lowest of the three short-term aquatic toxicity values from each of the three trophic levels was used as the point of departure for the derivation of the PNECaquatic (freshwater).