2-(4,6-bis-(2,4-dimethylphenyl)-1,3,5-triazin-2-yl)-5-(octyloxy)-phenol

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

other: Body responsible for the test

Title:

Unnamed

Year:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

412-440-4

EC Name:

-

Cas Number:

2725-22-6

Molecular formula:

C33-H39-N3-O2

IUPAC Name:

2-[4,6-bis(2,4-dimethylphenyl)-1,3,5-triazin-2-yl]-5-(octyloxy)phenol

Method

Target gene:

Not specified

Species / strain

Species / strain / cell type:

bacteria, other: Salmonella typhimurium, TA98, TA100, TA1535, and TA1538

Metabolic activation:

with and without

Metabolic activation system:

Liver microsomal enzymes from male Spraque-Dawley rats

Test concentrations with justification for top dose:

Concentration range in the main test (with metabolic activation): 667...10000 μg/plate
Concentration range in the main test (without metabolic activation): 667...10000 μg/plate

Vehicle / solvent:

Solvent: Acetone

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

yes

Remarks:

Acetone

True negative controls:

not specified

Positive controls:

not specified

Positive control substance:

not specified

Details on test system and experimental conditions:

Concentration of the test substance resulting in precipitation: 667 μg/plate

Rationale for test conditions:

not specified

Evaluation criteria:

not specified

Statistics:

not specified

Results and discussion

Test results

Test results 1

Species / strain:

other: as specified above

Metabolic activation:

with

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

5000 μg/plate

Test results 2

Species / strain:

other: as specified above

Metabolic activation:

without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

>5000 μg/plate

Test results 3

Species / strain:

other: as specified above

Metabolic activation:

with

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

>10000 μg/plate

Test results 4

Species / strain:

other: as specified above

Metabolic activation:

without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

>10000 μg/plate

Additional information on results:

Observations:
The vihicle control value for tester strain TA98 in the presence of microsomal enzymes was outside the acceptable range. Therefore, tester strain TA98 was retested in the presence of microsomal enzymes.

Remarks on result:

other: other: preliminary test

Applicant's summary and conclusion

Conclusions:

Gene mutation test was performed according to OECD Guideline 471 (Bacterial Reverse Mutation Assay) to determine the mutagenic nature of the test chemical. No cytotoxicity was observed till 10000 μg/plate dose range. Thus the test chemical was considered to be not mutagenic in nature.

Executive summary:

Gene mutation test was performed according to OECD Guideline 471 (Bacterial Reverse Mutation Assay) to determine the mutagenic nature of the test chemical. Salmonella typhimurium, TA98, TA100, TA1535, and TA1538 starins were used with and without metabolic activation system  obtained from Liver microsomal enzymes from male Spraque-Dawley rats. Concentration range in the main test (with metabolic activation): 667 to 10000 μg/plate and Concentration range in the main test (without metabolic activation): 667 to 10000 μg/plate were used. No cytotoxicity was observed till 10000 μg/plate dose range. Thus the test chemical was considered to be not mutagenic in nature.