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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
other: Body responsible for the test
Title:
Unnamed
Year:
1993

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
-
EC Number:
412-440-4
EC Name:
-
Cas Number:
2725-22-6
Molecular formula:
C33-H39-N3-O2
IUPAC Name:
2-[4,6-bis(2,4-dimethylphenyl)-1,3,5-triazin-2-yl]-5-(octyloxy)phenol
Details on test material:
Nature of substance: powder

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White

Test system

Vehicle:
not specified
Controls:
not specified
Amount / concentration applied:
100 mg
Duration of treatment / exposure:
4 hrs
Observation period (in vivo):
72 hrs
Duration of post- treatment incubation (in vitro):
not specified
Number of animals or in vitro replicates:
9 animals used
Details on study design:
not specified

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
conjunctivae score
Basis:
mean
Remarks:
overall at 24, 48 and 72 h
Time point:
24/48/72 h
Score:
1.67
Max. score:
1.67
Remarks on result:
other: Max. duration: 48 h; Max. value at end of observation period: 0
Irritation parameter:
chemosis score
Basis:
mean
Remarks:
overall at 24, 48 and 72 h
Time point:
24/48/72 h
Score:
0.67
Max. score:
0.67
Remarks on result:
other: Max. duration: 48 h; Max. value at end of observation period: 0
Irritation parameter:
cornea opacity score
Basis:
mean
Remarks:
overall at 24, 48 and 72 h
Time point:
24/48/72 h
Score:
0.16
Max. score:
0.16
Remarks on result:
other: Max. duration: 24 h; Max. value at end of observation period: 0
Irritation parameter:
iris score
Basis:
mean
Remarks:
overall at 24, 48 and 72 h
Time point:
24/48/72 h
Score:
0
Max. score:
0
Remarks on result:
other: Max. duration: 0 h; Max. value at end of observation period: 0
Irritant / corrosive response data:
Reversibility of any observed effect: Changes fully reversible within 3 days
Other effects:
Conjunctival irritation including blisterine in all but one animal after test article administration

Applicant's summary and conclusion

Interpretation of results:
other: Not irritating
Conclusions:
Eye irritation study was performed according to OECD Guideline 405 (Acute Eye Irritation / Corrosion) on rabbits. 9 New Zealand White rabbits were used in the study and the test chemical was applied at a concentration of 100 mg/kg bw for 4 hours. conjunctivae score of 1.67 was observed after 48 hrs of study, chemosis score of 0.67 was observed after 48 hrs of study, cornea opacity score of 0.16 was observed after 24 hrs of study and the iris score was 0 throughout the observation period. Changes were fully reversible within 3 days. As the toxic effects of the test chemical were reversible within 3 days, it was considered to be ‘not irritating’ to eyes in nature.
Executive summary:

Eye irritation study was performed according to OECD Guideline 405 (Acute Eye Irritation / Corrosion) on rabbits. 9 New Zealand White rabbits were used in the study and the test chemical was applied at a concentration of 100 mg/kg bw for 4 hours. conjunctivae score of 1.67 was observed after 48 hrs of study, chemosis score of 0.67 was observed after 48 hrs of study, cornea opacity score of 0.16 was observed after 24 hrs of study and the iris score was 0 throughout the observation period. Changes were fully reversible within 3 days. As the toxic effects of the test chemical were reversible within 3 days, it was considered to be ‘not irritating’ to eyes in nature.

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