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Toxicological information

Additional toxicological data

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Administrative data

Endpoint:
additional toxicological information
Remarks:
Cytotoxicity
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Drinking “Vodka” or vodka – This is a question
Author:
Izabela Rusiecka, Iwona Gągało, Jacek Sein Anand, Daria Schetz, WojciechWaldman
Year:
2016
Bibliographic source:
Toxicology in Vitro 36 (2016) 66–70

Materials and methods

Principles of method if other than guideline:
The direct effect of 2M2B on human tissuewas evaluated and compared to that of two other alcohols (ethanol, 3-´methyl-1-butanol)
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Name of the test material (as cited in study report): isoamyl alcohol/3-methyl-1-butanol
Specific details on test material used for the study:
no further details given

Results and discussion

Any other information on results incl. tables

The impact of each alcohol on HEK293 cell survivalwas further confirmed by the calculation of pIC50 and the values are as follows: 2M2B – 1.699, ethanol – 2.025, 3M1B – 0.884.

The results of MTT assay presented here show that the toxicity of 2M2B was comparable to that of ethanol, but lower than that observed after incubation with 3M1B. The drops in cell viability caused by the usage of the former two alcohols did not exceed 20%, and those caused by the latter were within the range of 40–80%.

Applicant's summary and conclusion