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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The test was performed in 1973 hence OECD Guidelines was not followed and analythical validation was not performed. However the test is roboust and well documented.
Qualifier:
no guideline followed
Principles of method if other than guideline:
The 4 -day dynamic toxicity of Dechlorane Plus 515 was determined in bluegill fingerlings at nominal concentrations up to 100 ppm.
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
yes
Details on test solutions:
prepared as suspension in acetone at up to 2% which was injected beneath the surface of the water at 90 seconds intervals
Test organisms (species):
other: bluegill fingerlings
Details on test organisms:
fingerlings with an average length of 35 - 55 mm
Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
none
Hardness:
164 - 184 ppm CaCO3
Test temperature:
18 °C
pH:
9.4 - 9.5
Dissolved oxygen:
8.7 - 10.0 ppm
Salinity:
alkalinity as ppm CaCO3 69 - 87
Nominal and measured concentrations:
0, 6.25, 12.5, 25.0, 50.0, 100.0 ppm nominal, not analytically verified.
Details on test conditions:
Injection of suspension in acetone at 90 seconds intervals beneath the water surface, due to lack of solubility, visible precipitations sank to the bottom of the tanks, no analytical determination of concentrations in water
Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
EC0
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
other: mortality and behaviour
Remarks on result:
other: no effects observed
Details on results:
No effects on mortality and behaviour at concentrations up to nominal 100 ppm, but visible precipitations accumulated at the bottom of the tanks
Results with reference substance (positive control):
none
Reported statistics and error estimates:
none reported
Validity criteria fulfilled:
no
Conclusions:
No toxicity observed but result not reliable due to low solubility of test substance.
Executive summary:

The 4 -day dynamic toxicity of Dechlorane Plus 515 was determined in bluegill fingerlings at nominal concentrations up to 100 ppm. No mortality and no effect on behaviour were observed, but the concentrations were not analytically verified, and visible precipitations accumulated at the bottom of the tanks. Due to the low water solubility of the test substance, the results is not conclusive.

Description of key information

The 4-day dynamic toxicity of Dechlorane Plus 515 was determined in bluegill fingerlings at nominal concentrations up to 100 ppm. No mortality and no effect on behaviour were observed, but the concentrations were not analytically verified, and visible precipitations accumulated at the bottom of the tanks. Due to the low water solubility of the test substance, the result is not conclusive.

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