1-ethynylcyclohexanol

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1962

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable publication which meets basic scientific principles.

Data source

Reference

Reference Type:

publication

Title:

Range-Finding Toxicity Data: List VI

Author:

Smyth HF, Carpenter CP, Weil CS et al.

Year:

1962

Bibliographic source:

Am Ind Hyg Assoc J 23: 95-107

Materials and methods

Principles of method if other than guideline:

Method: other: Smyth/Carpenter

GLP compliance:

no

Remarks:

prior to GLP

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

1-ethynylcyclohexanol

EC Number:

201-100-9

EC Name:

1-ethynylcyclohexanol

Cas Number:

78-27-3

Molecular formula:

C8H12O

IUPAC Name:

1-ethynylcyclohexanol

Test material form:

not specified

Test animals

Species:

rat

Strain:

other: albino

Sex:

male/female

Details on test animals or test system and environmental conditions:

Name of the test material: 1-Ethynylcyclohexanol

Administration / exposure

Route of administration:

inhalation

Type of inhalation exposure:

whole body

Vehicle:

air

Details on inhalation exposure:

INHALATION EXPOSURE AND TEST ATMOSPHERE
Concentrated vapor inhalation consisted of subjecting animals to a flowing stream of vapor-ladened air prepared by various styles of proportioning pumps. The vapor-air mixture was generated by passing 2.5 Liters/minute of dried air at room temperature through a fritted glass disc immersed to a depth of at least one inch in approximately 50 mL of the test chemical contained in a gas-washing bottle.

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

8 h

Concentrations:

Logarithmic series of vapor concentrations

No. of animals per sex per dose:

6 animals (male or female)

Control animals:

no

Details on study design:

INHALATION EXPOSURE TIMES
Rats were rapidly introduced by means of a drawer-type cage designed to minimize vapor loss. Inhalation of metered vapor concentrations were conducted were usually of four hours' duration unless slight toxicity enforces an eight-hour period (maximum period). For inhalation periods of ten, five and two minutes in duration, a static technique is used whereby 50 to 100 grams of material, spread over a shallow tray 200 square inches in area, is placed in a 120-Liter sealed chamber for at least 24 hours. This method was also employed for mixtures of liquids and for solids. Nominal concentrations were not analytically verified. They were in an essentially logarithmic series with a factor of two with a ratio of two extending from one-fourth to eight hours.

OBSERVATIONS
The period which permitted all rats to survive the two-week observation period was noticed. The concentration yielding fractional mortality was noticed. Where no fractional mortality was observed, usually both the concentration yielding no mortality and that yielding complete mortality were indicated.

Results and discussion

Effect levels

Dose descriptor:

LC0

Based on:

test mat.

Exp. duration:

8 h

Remarks on result:

other: saturated atmosphere

Mortality:

No mortality was observed.

Clinical signs:

other: No information provided

Body weight:

No information provided

Gross pathology:

No information provided

Any other information on results incl. tables

No mortality was observed when 6 rats were exposed for 8 hours to an atmosphere that had been saturated at room temperature with the volatile part of the compound.