Reaction mass of 3-[(2-aminoethyl)ammonio]propane-1-sulfonate and 3,3'-(ethane-1,2-diyldiammonio)dipropane-1-sulfonate and ethane-1,2-diamine

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

March - May 2008

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study with GLP.

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-[(2-aminoethyl)amino]propane-1-sulfonic acid

Cas Number:

14235-54-2

Molecular formula:

C5H14N2O3S

IUPAC Name:

3-[(2-aminoethyl)amino]propane-1-sulfonic acid

Details on test material:

- Name of test material: "3-[(2-AMINOETHYL)AMMONIO]PROPANE-1-SULFONATE" (EPS)
- Physical state: liquid
- Composition of test material, percentage of components: 48.5 % w/w 3-[(2-AMINOETHYL)AMMONIO]PROPANE-1-SULFONATE 51.5 % water
- Lot/batch No.: 06060423/101
- Stability under test conditions: not applicable
- Storage condition of test material: room temperature in the dark

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
none.

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge: sewage treatment works A-2500 Baden, which waste-water catchment is predominantly domestic
- Laboratory culture: On arrival in the laboratory and after sedimentation the clear supernatant was decanted and the sludge was filled up with tap water to its origin volume of 6 L to reduce the carbon content. The sample was aerated by means of a filtered compressed air before being used for the study.
- Pretreatment: none.
- Concentration of sludge: The concentration of the final suspended solids of the sludge in all vessels was nominally ca. 20 mg/L.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

93.9 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

15 mg/L

Based on:

other: carbon

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Composition of medium: mineral medium as in the guideline.
- Additional substrate: none.
- Solubilising agent (type and concentration if used): none.
- Test temperature: 22 +- 2 °C.
- pH: determined.
- pH adjusted: no
- Suspended solids concentration: 20 mg/L.
- Continuous darkness: yes.

TEST SYSTEM
- Culturing apparatus: 5 L flasks.
- Number of culture flasks/concentration: 2 negative control flasks, 2 test substance flasks, 1 positive control flask, 1 toxicity control flask.
- Method used to create aerobic conditions: Apparatus for carbon dioxide scrubbing and a mixture of CO2-free oxygen and CO2-free nitrogen, from gas cylinders, in the correct proportions (20 % O2: 80 % N2) were used.
- Test performed in closed vessels.

SAMPLING
- Sampling frequency: The CO2 evolution was determined on Days 1, 2, 4, 6, 8, 10, 14, 19, 24 and 28/29.
Day 0 was the day of the addition of the test substance to the medium.

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: yes, containing test substance and positive reference substance.
- Other: Positive reference substance.

STATISTICAL METHODS: no.

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

Preliminary study:

None.

Test performance:

No unusual observations were made.

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

19

Sampling time:

28 d

Remarks on result:

other: test substance

BOD5 / COD results

Results with reference substance:

Positive control: 70.4 % biodegradation after 6 days. The plateau of biodegradation was reached on about Day 10 and the degradation of the positive reference substance sodium benzoate exceeded the pass level of 60 % on about Day 5. The test is considered to be valid.
Toxicity control: 48.0 % biodegradation after 28 days. Degradation in the toxicity control, which contained sodium benzoate and the test substance, was not indicative of an inhibition of the microbial activity by the test substance.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: "3-[(2-AMINOETHYL)AMMONIO]PROPANE-1-SULFONATE" cannot be classified as readily biodegradable

Conclusions:

The biodegradability of "3-[(2-AMINOETHYL)AMMONIO]PROPANE-1-SULFONATE" was analysed by the determination of carbon dioxide evolution at frequent intervals over a 28 day period.
The method used was that described in Part C.4-C of the EC Commission Directive 92/69/EEC and in the OECD Guideline 301B.

Results
• The reference substance sodium benzoate was degraded by 87.6 % within 10 days.
• Degradation in the toxicity control, which contained sodium benzoate and the test substance, was not indicative of an inhibition of the microbial activity by the test substance.
• The carbon dioxide evolution from "3-[(2-AMINOETHYL)AMMONIO]PROPANE-1-SULFONATE" with a nominal concentration of 15 mg carbon/L reached 19.0 % after 28 days of incubation at mean temperatures of
22.5 °C.

Classification
According to the results of this study,"3-[(2-AMINOETHYL)AMMONIO]PROPANE-1-SULFONATE" cannot be classified as readily biodegradable.

Executive summary:

The biodegradability of "3-[(2-AMINOETHYL)AMMONIO]PROPANE-1-SULFONATE" was analysed by the determination of carbon dioxide evolution at frequent intervals over a 28 day period. The method used was that described in Part C.4-C of the EC Commission Directive 92/69/EEC and in the OECD Guideline 301B.

 

Results

·     The reference substance sodium benzoate was degraded by 87.6 % within 10 days.

·     Degradation in the toxicity control, which contained sodium benzoate and the test substance, was not indicative of an inhibition of the microbial activity by the test substance.

·     The carbon dioxide evolution from "3-[(2-AMINOETHYL)AMMONIO]PROPANE-1-SULFONATE" with a nominal concentration of 15 mg carbon/L reached 19.0 % after 28 days of incubation at mean temperatures of 22.5 °C.

Classification

According to the results of this study, "3-[(2-AMINOETHYL)AMMONIO]PROPANE-1-SULFONATE" cannot be classified as readily biodegradable.