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EC number: 411-280-2 | CAS number: 74091-64-8 MR-8A; MR-N2; NBDI
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- March 25, 1992 - December 11, 1992
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations:
1)Salinity control
2)control
3)6.25 mg/L
4)25 mg/L
5)100 mg/L
In duplicate, at t= 0; t=48 hr (before renewal); t=48 hr (after renewal) and at t=96 hr
- Sample storage conditions before analysis:
Samples for the analysis of NBDA were stored below -20 degrees Celsius until analysis. - Vehicle:
- no
- Details on test solutions:
- Preparation of the stock solution:
The stock solution was prepared twice during the test period. The amount of solid test chemical used was 2000 and 2002 mg on Sep. 27 and 29, 1992, respectively. The test chemical was added to 20 L reconstituted water. Before adding the test chemical, the pH of the water was adjusted to pH 2 by using HCL. The stock solution was stirred for 24 hours. During this period, NBDI dissociated completely. Before usage, the stock solution was adjusted to pH 7.8 with NaOH. The mean (nominal) concentration of the stock solution was 100.05 mg/L
Salinity control:
Reconstituted water adjusted to pH 2 with HCL and neutralised to pH 7.8 with NaOH - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow Trout
- Source: Forellenhof Fredelsloh, Mohringen, Germany
- Age at study initiation (mean): no data
- Length at study initiation (mean): 44.9 +/- 3.25 mm/fish
- Weight at study initiation (mean): 887.2 +/- 229.8 mg/fish
- Feeding during test: No
ACCLIMATION
- Acclimation period: 13 days - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- Not applicable
- Hardness:
- For all concentration levels and controls during the test period:
Min: 228 mg/L
Max: 267 mg/L
Mean: 240.29 mg/L - Test temperature:
- For all concentration levels and controls during the test period:
Min: 14.8 degrees Celsius
Max: 15.5 degrees Celsius
Mean: 15.06 degrees Celsius - pH:
- For all concentration levels and controls during the test period:
Min: 7.6
Max: 8.1
Mean: 7.81 - Dissolved oxygen:
- For all concentration levels and controls during the test period:
Min: 91%
Max: 96%
Mean: 93.57% - Salinity:
- Not applicable
- Nominal and measured concentrations:
- Salinity control; Control; 6.25 mg/L; 12.5 mg/L; 25 mg/L; 50 mg/L and 100 mg/L
Mean measured concentrations at 6.25, 25 and 100 mg NBDI/L: 5.6, 20 and 82 mg NBDA/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Stainless steel vessels (16 L)
- fill volume: 10 L
- Aeration: yes
- Renewal rate of test solution (frequency/flow rate): batchwise after 48 hours
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.887 g/L - Reference substance (positive control):
- not required
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 82 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- other: NBDA
- Details on results:
- No mortalities occurred.
The analytically determined concentrations of NBDA are shown in the Table under "Any other information on results incl. tables" - Results with reference substance (positive control):
- not applicable
- Sublethal observations / clinical signs:
Table 1. Analytical results: measured concentrations of NBDA during the semi-static test
nominal (mg NBDI/L) measured concentration (mg NBDA/L) 0, fresh 48, old 48, fresh 96, old mean 6.25 5.59 4.28 5.50 6.46 5.6 5.49 5.66 5.77 5.98 25 19.7 19.7 20.5 22.2 20 14.9 14.4 22.0 23.2 100 73.3 75.0 85.9 91.1 82 82.2 77.3 79.7 90.3 - Validity criteria fulfilled:
- yes
- Conclusions:
- The 96h-LC50 in Oncorhynchus mykiss was determined to be >82 mg NBDA/L
Reference
Description of key information
The 96h-LC50 in Oncorhynchus mykiss was determined to be >82 mg NBDA/L
Key value for chemical safety assessment
Additional information
A study was conducted with NBDA: test solutions were prepared by adding NBDI to test medium, and allowing the substance to dissociate completely. No fish died at an analytically determined mean concentration of 82 mg NBDA/L.
Value used for CSA: LC50 for freshwater fish: >82 mg NBDA/L
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